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Dystrophin constitutes 5% of membrane cytoskeleton in skeletal muscle
Journal article   Open access   Peer reviewed

Dystrophin constitutes 5% of membrane cytoskeleton in skeletal muscle

Kay Ohlendieck and Kevin P Campbell
FEBS letters, Vol.283(2), pp.230-234
06/03/1991
DOI: 10.1016/0014-5793(91)80595-T
PMID: 2044761
url
https://doi.org/10.1016/0014-5793(91)80595-TView
Published (Version of record) Open Access

Abstract

Dystrophin, which is absent in skeletal muscle of Duchenne muscular dystrophy patients, has not been considered to play a major structural role in the cell membrane of skeletal muscle because of its low abundance (∼ 0.002% of total muscle protein). Here, were have determined the relative abundance of dystrophin in a membrane cytoskeleton preparation and found that dystrophin constitutes approximately 5% of the total membrane cytoskeleton fraction of skeletal muscle sarcolemma. In addition, dystrophin can be removed from surcolemma by alkaline treatment. Thus, our results have demonstrated that dystrophin is a major component of the subsurcolemmal cytoskeleton in skeletal muscle and suggest that dystrophin could play a major structural role in the cell membrane of skeletal muscle.
Sarcolemma Membrane cytoskeleton Dystrophin Skeletal muscle

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