Logo image
Dystrophin-related protein is localized to neuromuscular junctions of adult skeletal muscle
Journal article   Open access   Peer reviewed

Dystrophin-related protein is localized to neuromuscular junctions of adult skeletal muscle

Kay Ohlendieck, James M Ervasti, Kiichiro Matsumura, Steven D Kahl, Cynthia J Leveille and Kevin P Campbell
Neuron (Cambridge, Mass.), Vol.7(3), pp.499-508
1991
DOI: 10.1016/0896-6273(91)90301-F
PMID: 1654951
url
https://doi.org/10.1016/0896-6273(91)90301-FView
Published (Version of record) Open Access

Abstract

Dystrophin-related protein (DRP) is an autosomal gene product with high homology to dystrophin. We have used highly specific antibodies to the unique C-terminal peptide sequences of DRP and dystrophin to examine the subcellular localization and biochemical properties of DRP in adult skeletal muscle. DRP is enriched in isolated sarcolemma from control and mdx mouse muscle, but is much less abundant than dystrophin. Immunofluorescence microscopy localized DRP almost exclusively to the neuromuscular junction region in rabbit and mouse skeletal muscle, as well as mdx mouse muscle and denervated mouse muscle. DRP is also present in normal size and abundance and localizes to the neuromuscular junction region in muscle from the dystrophic mouse model dy dy . Thus, DRP is a junction-specific membrane cytoskeletal protein that may play an important role in the organization of the postsynaptic membrane of the neuromuscular junction.

Details

Metrics

Logo image