Journal article
ECM1 Expression in Thyroid Tumors—A Comparison of Real-Time RT-PCR and IHC
The Journal of surgical research, Vol.149(1), pp.62-68
2008
DOI: 10.1016/j.jss.2007.10.014
PMID: 18374945
Abstract
Increased extracellular matrix 1 (ECM1) expression, as determined by real-time reverse transcriptase-polymerase chain reaction (RT-PCR), has been recently proposed as a novel and independent diagnostic marker for malignant thyroid nodules. However ECM1 protein expression has not been previously evaluated in thyroid tumors. Real-time RT-PCR is not widely available and this has important implications for the widespread use of the expression of this gene for diagnosis. The purpose of the current study is to evaluate and compare ECM1 expression by real-time RT-PCR with immunohistochemistry (IHC) as diagnostic aids in thyroid neoplasms.
ECM1 expression was studied in normal thyroid tissues (
n = 14) and primary thyroid tumors (19 benign, 30 malignant) using both techniques. The performance characteristics of ECM1 expression were examined with receiver operating characteristic curves and the resulting area under the curve.
ECM1 was highly expressed in thyroid carcinomas, compared with benign thyroid tissues, both at the mRNA (
P < 0.01) and protein (
P = 0.06) levels. However, ECM1 mRNA expression appeared to be a more sensitive marker of thyroid malignancy than IHC (AUC = 0.77 and 0.65, respectively). ECM1 expression by both methods was useful in identifying malignant follicular, but not Hürthle cell neoplasms.
We confirm that ECM1 expression has potential utility as an independent diagnostic marker for thyroid cancer, although, performance characteristics were lower than previously published. Furthermore, IHC was not as sensitive as real-time RT-PCR in identifying malignant lesions. Prospective studies are needed to further clarify the role of ECM1 expression as a diagnostic marker.
Details
- Title: Subtitle
- ECM1 Expression in Thyroid Tumors—A Comparison of Real-Time RT-PCR and IHC
- Creators
- Geeta Lal - Department of Surgery, Section of Surgical Oncology and Endocrine Surgery; University of Iowa Hospitals and Clinics, Iowa City, IowaLakshmi Padmanabha - Department of Surgery, Section of Surgical Oncology and Endocrine Surgery; University of Iowa Hospitals and Clinics, Iowa City, IowaRachael Nicholson - Department of Surgery, Section of Surgical Oncology and Endocrine Surgery; University of Iowa Hospitals and Clinics, Iowa City, IowaBrian J Smith - Department of Biostatistics; University of Iowa Hospitals and Clinics, Iowa City, IowaLurong Zhang - University of Rochester Medical Center; University of Iowa Hospitals and Clinics, Iowa City, IowaJames R Howe - Department of Surgery, Section of Surgical Oncology and Endocrine Surgery; University of Iowa Hospitals and Clinics, Iowa City, IowaRobert A Robinson - Department of Pathology; University of Iowa Hospitals and Clinics, Iowa City, IowaM. Sue O'Dorisio - Department of Pediatrics; University of Iowa Hospitals and Clinics, Iowa City, Iowa
- Resource Type
- Journal article
- Publication Details
- The Journal of surgical research, Vol.149(1), pp.62-68
- Publisher
- Elsevier Inc
- DOI
- 10.1016/j.jss.2007.10.014
- PMID
- 18374945
- ISSN
- 0022-4804
- eISSN
- 1095-8673
- Language
- English
- Date published
- 2008
- Academic Unit
- Oral Pathology, Radiology and Medicine; Stead Family Department of Pediatrics; Pathology; Biostatistics; Surgery; Holden Comprehensive Cancer Center
- Record Identifier
- 9983997997102771
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