Journal article
ELISA for human serum leucine-rich alpha-2-glycoprotein-1 employing cytochrome c as the capturing ligand
Journal of immunological methods, Vol.336(1), pp.22-29
2008
DOI: 10.1016/j.jim.2008.03.004
PMCID: PMC7094546
PMID: 18436231
Abstract
Leucine-rich alpha-2-glycoprotein-1 (LRG) is a serum glycoprotein of unknown function that has shown promise based on qualitative assessments as a biomarker for certain diseases including microbial infections and cancer. However, the lack of a quantitative assay for LRG has limited its application. Here an indirect enzyme-linked immunosorbent assay (ELISA) for quantifying LRG in human serum is described in which cytochrome
c is employed as the capturing ligand and a monoclonal antibody specific for LRG is used to detect the captured glycoprotein. Application of this assay in quantifying LRG in various patients' sera is demonstrated. The concentration of LRG in sera of control subjects as determined by this assay is approximately 50 μg/ml. Consistent with expectations from published reports, LRG was found to be significantly elevated in the sera of some patients with a bacterial infection (toxic shock syndrome, TSS). LRG was only slightly elevated in patients infected with the human immunodeficiency virus as compared to uninfected control subjects, while normal levels of LRG were observed in patients with non-infectious diseases (inflammatory arthritis and neurological disorders, primarily Parkinson's disease). Although LRG and C-reactive protein (CRP) are both produced by the liver and are classified as acute-phase proteins, there was no significant correlation between the levels of LRG and CRP in the sera of the patients. Thus, LRG and CRP measurements are non-redundant and indicate different physiological contexts. The ELISA described in this report should be useful to further assess serum LRG as a biomarker for clinical diagnostics.
Details
- Title: Subtitle
- ELISA for human serum leucine-rich alpha-2-glycoprotein-1 employing cytochrome c as the capturing ligand
- Creators
- Starchild Weivoda - Department of Microbiology and Center for Immunology, University of Minnesota, United StatesJohn D Andersen - Department of Laboratory Medicine and Pathology, University of Minnesota, United StatesAunica Skogen - Department of Microbiology and Center for Immunology, University of Minnesota, United StatesPatrick M Schlievert - Department of Microbiology and Center for Immunology, University of Minnesota, United StatesDonna Fontana - Arthritis and Rheumatology Consultants, Edina, MN 55435, United StatesTimothy Schacker - Department of Medicine, University of Minnesota, Minneapolis, MN 55455, United StatesPaul Tuite - Department of Neurology, University of Minnesota, Minneapolis, MN 55455, United StatesJanet M Dubinsky - Department of Neuroscience, University of Minnesota, Minneapolis, MN 55455, United StatesRonald Jemmerson - Department of Microbiology and Center for Immunology, University of Minnesota, United States
- Resource Type
- Journal article
- Publication Details
- Journal of immunological methods, Vol.336(1), pp.22-29
- DOI
- 10.1016/j.jim.2008.03.004
- PMID
- 18436231
- PMCID
- PMC7094546
- NLM abbreviation
- J Immunol Methods
- ISSN
- 0022-1759
- eISSN
- 1872-7905
- Publisher
- Elsevier B.V
- Language
- English
- Date published
- 2008
- Academic Unit
- Microbiology and Immunology; Internal Medicine
- Record Identifier
- 9984001214902771
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