Journal article
ENZYMATIC PREPARATION OF HEPARIN DISACCHARIDES AS BUILDING BLOCKS IN GLYCOSAMINOGLYCAN SYNTHESIS
Preparative biochemistry & biotechnology, Vol.31(2), pp.113-124
04/30/2001
DOI: 10.1081/PB-100103377
PMID: 11426699
Abstract
Pharmaceutical heparin and heparan sulfate, isolated from a side-stream of a commercial heparin manufacturing process, have been enzymatically depolymerzed with heparin lyases obtained from Flavobacterium heparinun. Heparin afforded a trisulfated disaccharide product that was recovered from the reaction mixture using gel permeation chromatography.
Heparan sulfate afforded unsulfated disaccharide that was conveniently recovered from the product mixture by ion exchange chromatography. Both disaccharides were obtained in gram amounts at 90% or higher purity. Both enzymatically prepared disaccharides were chemically protected to prepare building blocks required for the future chemical synthesis of therapeutically valuable heparin oligosaccharides.
Details
- Title: Subtitle
- ENZYMATIC PREPARATION OF HEPARIN DISACCHARIDES AS BUILDING BLOCKS IN GLYCOSAMINOGLYCAN SYNTHESIS
- Creators
- Yeong Shik Kim - Seoul National UniversityCharuwan Thanawiroon - University of IowaHélène G. Bazin - University of IowaRobert J. Kerns - Wayne State UniversityRobert J. Linhardt - University of Iowa
- Resource Type
- Journal article
- Publication Details
- Preparative biochemistry & biotechnology, Vol.31(2), pp.113-124
- Publisher
- Taylor & Francis Group
- DOI
- 10.1081/PB-100103377
- PMID
- 11426699
- ISSN
- 1082-6068
- eISSN
- 1532-2297
- Language
- English
- Date published
- 04/30/2001
- Academic Unit
- Pharmaceutical Sciences and Experimental Therapeutics; Medicinal and Natural Products Chemistry
- Record Identifier
- 9984366032002771
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