Journal article
ERK Activation and Nuclear Signaling Induced by the Loss of Cell/Matrix Adhesion Stimulates Anchorage-Independent Growth of Ovarian Cancer Cells
Journal of cellular biochemistry, Vol.105(3), pp.875-884
10/15/2008
DOI: 10.1002/jcb.21889
PMID: 18726893
Abstract
Ovarian cancer metastasis involves the sloughing of epithelial cells from the ovary into the peritoneal cavity, where the cells can survive and proliferate in peritoneal ascites under anchorage-in dependent conditions. For normal epithelial cells and fibroblasts, cell adhesion to the extracellular matrix is required to prevent apoptosis and for proper activation and nuclear signaling of the ERK MAP kinase. The mechanisms of ERK regulation by adhesion have been determined by our lab and others. In this report, we elucidate a novel means of ERK regulation by cellular adhesion in ovarian cancer cells. We demonstrate that ERK and its activator MEK are robustly stimulated after cell detachment from a substratum in several ovarian cancer cell lines, but not a benign ovarian cell line, independent of serum and FAK or PAK activity. MEK and ERK activation was sustained for 48 h after detachment, while activation by serum or growth factors in adherent cells was transient. Re-attachment of suspended ovarian cells to fibronectin restored basal levels of MEK and ERK activity. ERK activity in suspended cells was dynamically controlled through an autocrine stimulatory pathway and prevalent phosphatase activity. Suspended cells demonstrated higher levels of ERK nuclear signaling to Elk I compared to adherent cells. Inhibition of ERK activation with the MEK inhibitor U0126 had minor effects on adherent cell growth, but greatly decreased growth in soft agar. These data demonstrate a unique regulation of ERK by cellular adhesion and suggest a mechanism by which ERK may regulate anchorage-independent growth of metastatic ovarian cancer cells. J. Cell. Biochem. 105: 875-884, 2008. (C) 2008 Wiley-Liss, Inc.
Details
- Title: Subtitle
- ERK Activation and Nuclear Signaling Induced by the Loss of Cell/Matrix Adhesion Stimulates Anchorage-Independent Growth of Ovarian Cancer Cells
- Creators
- Adnan Al Ayoubi - University of Iowa, Cardiothoracic SurgeryAdel Tarcsafalvi - University of VirginiaHui Zheng - Medical University of South CarolinaWayne Sakati - Medical University of South CarolinaScott T. Eblen - Medical University of South Carolina
- Resource Type
- Journal article
- Publication Details
- Journal of cellular biochemistry, Vol.105(3), pp.875-884
- Publisher
- Wiley
- DOI
- 10.1002/jcb.21889
- PMID
- 18726893
- ISSN
- 0730-2312
- eISSN
- 1097-4644
- Number of pages
- 10
- Grant note
- Women's Oncology Research Fund of the University of Virginia Cancer Center W81XWH-04-1-0100 / Department of Defense; United States Department of Defense
- Language
- English
- Date published
- 10/15/2008
- Academic Unit
- Cardiothoracic Surgery
- Record Identifier
- 9984701723902771
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