Journal article
ESCRT ubiquitin-binding domains function cooperatively during MVB cargo sorting
The Journal of cell biology, Vol.185(2), pp.213-224
04/20/2009
DOI: 10.1083/jcb.200811130
PMCID: PMC2700381
PMID: 19380877
Abstract
Ubiquitin (Ub) sorting receptors facilitate the targeting of ubiquitinated membrane proteins into multivesicular bodies (MVBs). Ub-binding domains (UBDs) have been described in several endosomal sorting complexes required for transport (ESCRT). Using available structural information, we have investigated the role of the multiple UBDs within ESCRTs during MVB cargo selection. We found a novel UBD within ESCRT-I and show that it contributes to MVB sorting in concert with the known UBDs within the ESCRT complexes. These experiments reveal an unexpected level of coordination among the ESCRT UBDs, suggesting that they collectively recognize a diverse set of cargo rather than act sequentially at discrete steps.
Details
- Title: Subtitle
- ESCRT ubiquitin-binding domains function cooperatively during MVB cargo sorting
- Creators
- S. Brookhart Shields - University of IowaAndrea J. Oestreich - Department of Biochemistry and Molecular Biology, Mayo Clinic College of Medicine, Rochester, MN 55905Stanley Winistorfer - University of IowaDoris Nguyen - Department of Biochemistry and Molecular Biology, Mayo Clinic College of Medicine, Rochester, MN 55905Johanna A. Payne - Department of Biochemistry and Molecular Biology, Mayo Clinic College of Medicine, Rochester, MN 55905David J. Katzmann - Department of Biochemistry and Molecular Biology, Mayo Clinic College of Medicine, Rochester, MN 55905Robert Piper - University of Iowa
- Resource Type
- Journal article
- Publication Details
- The Journal of cell biology, Vol.185(2), pp.213-224
- DOI
- 10.1083/jcb.200811130
- PMID
- 19380877
- PMCID
- PMC2700381
- NLM abbreviation
- J Cell Biol
- ISSN
- 0021-9525
- eISSN
- 1540-8140
- Publisher
- Rockefeller Univ Press
- Number of pages
- 12
- Grant note
- R01 GM073024 / NIGMS NIH HHS; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Institute of General Medical Sciences (NIGMS) R01GM073024 / NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Institute of General Medical Sciences (NIGMS)
- Language
- English
- Date published
- 04/20/2009
- Academic Unit
- Molecular Physiology and Biophysics; Medicine Administration; Internal Medicine
- Record Identifier
- 9984297494402771
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