EST-based gene discovery in pig: virtual expression patterns and comparative mapping to human

Christopher K Tuggle; Jon A Green; Carolyn Fitzsimmons; Rami Woods; Randall S Prather; Sergei Malchenko; Bento M Soares; Tamara Kucaba; Keith Crouch; Christina Smith; Dylan Tack; Natalie Robinson; Brian O'Leary; Todd Scheetz; Thomas Casavant; Daniel Pomp; Brad J Edeal; Yuandan Zhang; Max F Rothschild; Kevin Garwood; William Beavis

doi:10.1007/s00335-002-2263-7

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EST-based gene discovery in pig: virtual expression patterns and comparative mapping to human

Journal article

Peer reviewed

EST-based gene discovery in pig: virtual expression patterns and comparative mapping to human

Christopher K Tuggle, Jon A Green, Carolyn Fitzsimmons, Rami Woods, Randall S Prather, Sergei Malchenko, Bento M Soares, Tamara Kucaba, Keith Crouch, Christina Smith, …

Mammalian genome, Vol.14(8), pp.565-579

08/2003

DOI: 10.1007/s00335-002-2263-7

PMID: 12925889

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Abstract

A molecular understanding of porcine reproduction is of biological interest and economic importance. Our Midwest Consortium has produced cDNA libraries containing the majority of genes expressed in major female reproductive tissues, and we have deposited into public databases 21,499 expressed sequence tag (EST) gene sequences from the 3' end of clones from these libraries. These sequences represent 10,574 different genes, based on sequence comparison among these data, and comparison with existing porcine ESTs and genes indicate as many as 4652 of these EST clusters are novel. In silico analysis identified sequences that are expressed in specific pig tissues or organs and confirmed the broad expression in pig for many genes ubiquitously expressed in human tissues. Furthermore, we have developed computer software to identify sequence similarity of these pig genes with their human counterparts, and to extract the mapping information of these human homologues from genome databases. We demonstrate the utility of this software for comparative mapping by localizing 61 genes on the porcine physical map for Chromosomes (Chrs) 5, 10, and 14.

Sus scrofa - genetics

Gene Expression

Algorithms

Animals

Gene Library

Base Sequence

Humans

Hybrid Cells

Molecular Sequence Data

Chromosome Mapping

Sequence Analysis, DNA

Expressed Sequence Tags

Details

Title: Subtitle: EST-based gene discovery in pig: virtual expression patterns and comparative mapping to human
Creators: Christopher K Tuggle - Center for Integrated Animal Genomics and Department of Animal Science, Iowa State University, 2255 Kildee Hall, Ames, Iowa 50011, USA. cktuggle@iastate.edu
Jon A Green
Carolyn Fitzsimmons
Rami Woods
Randall S Prather
Sergei Malchenko
Bento M Soares
Tamara Kucaba
Keith Crouch
Christina Smith
Dylan Tack
Natalie Robinson
Brian O'Leary
Todd Scheetz
Thomas Casavant
Daniel Pomp
Brad J Edeal
Yuandan Zhang
Max F Rothschild
Kevin Garwood
William Beavis
Resource Type: Journal article
Publication Details: Mammalian genome, Vol.14(8), pp.565-579
Publisher: United States
DOI: 10.1007/s00335-002-2263-7
PMID: 12925889
ISSN: 0938-8990
eISSN: 1432-1777
Language: English
Date published: 08/2003
Academic Unit: Roy J. Carver Department of Biomedical Engineering; Electrical and Computer Engineering; Pathology; Ophthalmology and Visual Sciences
Record Identifier: 9983979965902771

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