Journal article
ETn insertion in the mouse Adcy1 gene: transcriptional and phylogenetic analyses
Mammalian genome, Vol.11(2), pp.97-103
02/2000
DOI: 10.1007/s003350010020
PMID: 10656922
Abstract
Early retrotransposons (ETn) are murine transposable elements, bearing some structural similarity to integrated proviruses, and can be insertional mutagens. We have recently identified the causative mutation of the barrelless (Adcy1
brl
) phenotype as an integration of a 5.7-kb ETn in an intron of the adenylyl cyclase type I (Adcy1) gene. In the present study, Northern blot analysis shows that the ETn insertion results in loss of the normal Adcy1 transcript, a finding consistent with the loss-of-function Adcy1
brl
mutation, and generation of shorter transcripts. These aberrant transcripts are the products of abnormal RNA splicing and termination owing to the inserted sequence, and transcription initiation within the 3′ long terminal repeat (LTR) of the ETn. The DNA sequences of the LTRs were compared in phylogenetic analyses with LTRs from 22 other ETn-related sequences. Three distinct families of ETn sequences can be identified on the basis of their LTRs. The ETn found in Adcy1
brl
is a member of a family that includes all classified ETn elements known to have recently transposed. Further, of the four known solitary (solo) LTRs, we have identified two that show evidence of recombination between LTRs from different ETn families.
Details
- Title: Subtitle
- ETn insertion in the mouse Adcy1 gene: transcriptional and phylogenetic analyses
- Creators
- Wey L Leong - Department of Pathology, Faculty of Medicine, Dalhousie University, Halifax, NS, Canada B3H 4H7 CAMelanie J Dobson - Department of Biochemistry, Faculty of Medicine, Dalhousie University, Halifax, NS, Canada B3H 4H7 CAJohn M Logsdon Jr - Department of Biochemistry, Faculty of Medicine, Dalhousie University, Halifax, NS, Canada B3H 4H7 CARaja M Abdel-Majid - Department of Anatomy & Neurobiology, Faculty of Medicine, Dalhousie University, Halifax, NS, Canada B3H 4H7 CALeonard C Schalkwyk - Max-Planck-Institut fuer Molekulare Genetik, abteilung Lehrach, Berlin-Dahlem, Germany DEDuane L Guernsey - Department of Pathology, Faculty of Medicine, Dalhousie University, Halifax, NS, Canada B3H 4H7 CAPaul E Neumann - Department of Pathology, Faculty of Medicine, Dalhousie University, Halifax, NS, Canada B3H 4H7 CA
- Resource Type
- Journal article
- Publication Details
- Mammalian genome, Vol.11(2), pp.97-103
- Publisher
- Springer-Verlag
- DOI
- 10.1007/s003350010020
- PMID
- 10656922
- ISSN
- 0938-8990
- eISSN
- 1432-1777
- Language
- English
- Date published
- 02/2000
- Academic Unit
- Biology
- Record Identifier
- 9984217420102771
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