EWI-2 is a major CD9 and CD81 partner and member of a novel Ig protein subfamily

Christopher S Stipp; Tatiana V Kolesnikova; Martin E Hemler

doi:10.1074/jbc.M107338200

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EWI-2 is a major CD9 and CD81 partner and member of a novel Ig protein subfamily

Journal article

Open access

Peer reviewed

EWI-2 is a major CD9 and CD81 partner and member of a novel Ig protein subfamily

Christopher S Stipp, Tatiana V Kolesnikova and Martin E Hemler

The Journal of biological chemistry, Vol.276(44), pp.40545-40554

11/02/2001

DOI: 10.1074/jbc.M107338200

PMID: 11504738

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Abstract

A novel Ig superfamily protein, EWI-2, was co-purified with tetraspanin protein CD81 under relatively stringent Brij 96 detergent conditions and identified by mass spectrometric protein sequencing. EWI-2 associated specifically with CD9 and CD81 but not with other tetraspanins or with integrins. Immunodepletion experiments indicated that EWI-2-CD9/CD81 interactions are highly stoichiometric, with approximately 70% of CD9 and CD81 associated with EWI-2 in an embryonic kidney cell line. The EWI-2 molecule was covalently cross-linked (in separate complexes) to both CD81 and CD9, suggesting that association is direct. EWI-2 is part of a novel Ig subfamily that includes EWI-F (F2alpha receptor regulatory protein (FPRP), CD9P-1), EWI-3 (IgSF3), and EWI-101 (CD101). All four members of this Ig subfamily contain a Glu-Trp-Ile (EWI) motif not seen in other Ig proteins. As shown previously, the EWI-F molecule likewise forms highly proximal, specific, and stoichiometric complexes with CD9 and CD81. Human and murine EWI-2 protein sequences are 91% identical, and transcripts in the two species are expressed in virtually every tissue tested. Thus, EWI-2 potentially contributes to a variety of CD9 and CD81 functions seen in different cell and tissue types.

Amino Acid Sequence

Cell Line

Molecular Sequence Data

Tetraspanin 28

Immunoglobulins - genetics

DNA Primers

Antigens, CD - metabolism

Immunoglobulins - metabolism

Membrane Proteins

Sequence Homology, Amino Acid

Immunoglobulins - chemistry

Animals

Base Sequence

Membrane Glycoproteins

Tumor Cells, Cultured

Tetraspanin-29

Details

Title: Subtitle: EWI-2 is a major CD9 and CD81 partner and member of a novel Ig protein subfamily
Creators: Christopher S Stipp - Dana-Farber Cancer Institute and the Department of Pathology, Harvard Medical School, Boston, Mssachusetts 02115, USA
Tatiana V Kolesnikova
Martin E Hemler
Resource Type: Journal article
Publication Details: The Journal of biological chemistry, Vol.276(44), pp.40545-40554
DOI: 10.1074/jbc.M107338200
PMID: 11504738
NLM abbreviation: J Biol Chem
ISSN: 0021-9258
eISSN: 1083-351X
Publisher: United States
Grant note: GM38903 / NIGMS NIH HHS
Language: English
Date published: 11/02/2001
Academic Unit: Molecular Physiology and Biophysics; Biology
Record Identifier: 9983991989702771

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