Journal article
EYA1 expression in the developing inner ear
Annals of otology, rhinology & laryngology, Vol.114(11), pp.853-858
11/2005
DOI: 10.1177/000348940511401108
PMID: 16358604
Abstract
We sought to determine the developmental anatomy and EYA1 protein distribution in the inner ear of Xenopus laevis.
Xenopus laevis embryos were stained with monoclonal antibodies and imaged with confocal microscopy.
At stage 27, the otocyst fully forms, with strong tubulin staining of early sensory cells at its ventromedial aspect. Neuronal ingrowth follows at stage 33/34. At stage 50, the semicircular canals are complete. EYA1 localizes to the anterior aspect of the otocyst from stages 37 to 44. By stage 50, EYA1 distribution is localized primarily to the sensory maculae and the endolymphatic duct of the developing inner ear.
Whole mount confocal imaging of the developing Xenopus inner ear delineates the exact timing of otic development, sensory cell differentiation, and innervation. EYA1 protein expression has a distinct distribution pattern at the anterior aspect of the developing otocyst in stages 41 and 44. Later stages have a more localized pattern, in which EYA1 is detected only in the sensory epithelium and endolymphatic duct. This specific pattern of expression indicates a possible role in the determination of the anterior-posterior orientation of the inner ear, as well as a later role in sensory cell differentiation.
Details
- Title: Subtitle
- EYA1 expression in the developing inner ear
- Creators
- Brian C Bane - Department of Otolaryngology-Head and Neck Surgery University of Iowa Roy J. and Lucille A. Carver College of Medicine, Iowa City, Iowa, USAJana M Van RybroekSandra J KolkerDaniel L WeeksJose M Manaligod
- Resource Type
- Journal article
- Publication Details
- Annals of otology, rhinology & laryngology, Vol.114(11), pp.853-858
- Publisher
- United States
- DOI
- 10.1177/000348940511401108
- PMID
- 16358604
- ISSN
- 0003-4894
- eISSN
- 1943-572X
- Language
- English
- Date published
- 11/2005
- Academic Unit
- Stead Family Department of Pediatrics; Anesthesia; Biochemistry and Molecular Biology; Otolaryngology
- Record Identifier
- 9984006337402771
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