Journal article
Early detection of bleomycin-induced lung injury in rat using indium-111-labeled antibody directed against intercellular adhesion molecule-1
The Journal of nuclear medicine (1978), Vol.39(4), pp.723-728
1998
PMID: 9544689
Abstract
We have investigated whether an (111)In-labeled mouse monoclonal antibody to rat intercellular adhesion molecule-1 ((111)In*aICAM-1) could detect lung injury early in rats treated with bleomycin.
Methods: Rats received an intravenous injection of either (111)In*aICAM-1 or (111)In-labeled normal mouse IgG ((111)In*nmIgG) and were imaged and killed 24 hr later. Lung injury was induced by an intratracheal injection of bleomycin 4 or 24 hr before the rats were killed. After death, tissue was removed and activity was measured, lungs were cryostat-sectioned to detect the presence of ICAM-1 by immunofluorescence, and the up-regulation of LFA-1alpha was examined on blood polymorphonuclear leukocytes (PMNs) using fluorescence-activated cell-sorter (FACS) analysis.
Results: In rats injected with (111)In*aICAM-1, the percent injected dose/organ in lungs both at 4 and 24 hr postbleomycin increased significantly compared to the values in either uninjured rats or rats that received (111)In*nmIgG. At 4 and 24 hr postinjury, the target-to-blood (T/B) ratio was 8/1 and 6/1, respectively. For (111)In*nmIgG, the T/B ratio at 4 hr was 0.5/1 and 0.4/1 at 24 hr. In (111)In*aICAM-1 rats injured at 4 or 24 hr, images could easily be distinguished from uninjured rats. All images of (111)In*nmIgG rats showed only cardiac blood-pool and liver activity with little lung activity. Lung ICAM-1 immunofluorescence intensity increased in the bleomycin-treated samples compared to uninjured lungs. Expression of LFA-1alpha on PMNs increased 19% and 210% at 4 hr and 24 hr postinjury, respectively, compared to control values.
Conclusion: Biodistribution and imaging data demonstrate that (111)In*aICAM-1 can detect early acute bleomycin-induced lung injury. Immunofluorescence and FACS data suggest that (111)In*ICAM-1 uptake is a specific process. This antibody has potential as an early radionuclide detector of acute inflammations.
Details
- Title: Subtitle
- Early detection of bleomycin-induced lung injury in rat using indium-111-labeled antibody directed against intercellular adhesion molecule-1
- Creators
- R. E WEINER - Department of Radiology, VA Medical Center, Newington, Connecticut, United StatesD. E SASSO - Department of Radiology, VA Medical Center, Newington, Connecticut, United StatesM. A GIONFRIDDO - Department of Radiology, VA Medical Center, Newington, Connecticut, United StatesS. I SYRBU - Department of Radiology, VA Medical Center, Newington, Connecticut, United StatesH. M SMILOWITZ - Department of Radiology, VA Medical Center, Newington, Connecticut, United StatesJ VENTO - Department of Radiology, VA Medical Center, Newington, Connecticut, United StatesR. S THRALL - Department of Radiology, VA Medical Center, Newington, Connecticut, United States
- Resource Type
- Journal article
- Publication Details
- The Journal of nuclear medicine (1978), Vol.39(4), pp.723-728
- Publisher
- Society of Nuclear Medicine; Reston, VA
- PMID
- 9544689
- ISSN
- 0161-5505
- eISSN
- 1535-5667
- Language
- English
- Date published
- 1998
- Academic Unit
- Pathology
- Record Identifier
- 9984047896902771
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