Journal article
Early detection of oleic acid-induced lung injury in rats using 111In-labeled anti-rat intercellular adhesion molecule-1
The Journal of nuclear medicine (1978), Vol.42(7), pp.1109-1115
2001
PMID: 11438635
Abstract
Previous study of the bleomycin-induced lung injury model suggested that (111)In-labeled antirat intercellular adhesion molecule-1 (aICAM-1) might be a useful acute respiratory distress syndrome (ARDS) diagnostic agent. We further investigated the ability of (111)In-aICAM-1 to detect inflammation in another ARDS lung injury model.
Methods: (111)In-labeled rat polymorphonuclear leukocytes (PMNs), (111)In-aICAM-1, (111)In-labeled normal mouse IgG (nmIgG), and (111)In-labeled rat serum albumin (RSA) were injected into rats 18-24 h before kill. Biodistributions, scintigraphic images, and lung ICAM-1 upregulation were obtained in uninjured rats and in rats after injury with oleic acid.
Results: (111)In-RSA and (111)In-nmIgG localized in inflamed lung at 5 min postinjury (PI). (111)In-PMN uptake increased significantly only at 24 h PI. (111)In-aICAM-1 localization increased significantly (30%-60%) at 1 h PI and remained elevated up to 24 h PI. Lung/blood ratios (L/B) at 1 and 4 h PI were very low (<0.6) for (111)In-nmIgG and (111)In-PMN rats; however, for (111)In-aICAM-1 rats, they were >1 and 25%-60% higher than those for the control samples. A low L/B suggests poor inflammation detection on the images. Images and region-of-interest analysis confirmed that only (111)In-aICAM-1 could distinguish inflamed lungs at 4 h PI. ICAM-1 was upregulated at 4 and 24 h PI.
Conclusion: In this model, (111)In-aICAM-1 detected lung inflammation very early in the course of the disease. These results support the suggestion that (111)In-aICAM-1 could be a very early, highly specific ARDS diagnostic agent and may be useful to detect a wide range of inflammations.
Details
- Title: Subtitle
- Early detection of oleic acid-induced lung injury in rats using 111In-labeled anti-rat intercellular adhesion molecule-1
- Creators
- Ronald E WEINER - Department of Radiology, VA Medical Center, Newington, Connecticut, United StatesDaniel E SASSO - Department of Radiology, VA Medical Center, Newington, Connecticut, United StatesMaria A GIONFRIDDO - Department of Radiology, VA Medical Center, Newington, Connecticut, United StatesRoger S THRALL - Department of Radiology, VA Medical Center, Newington, Connecticut, United StatesSergei SYRBU - Department of Radiology, VA Medical Center, Newington, Connecticut, United StatesHenry M SMILOWITZ - Department of Radiology, VA Medical Center, Newington, Connecticut, United StatesJohn VENTO - Department of Radiology, VA Medical Center, Newington, Connecticut, United States
- Resource Type
- Journal article
- Publication Details
- The Journal of nuclear medicine (1978), Vol.42(7), pp.1109-1115
- Publisher
- Society of Nuclear Medicine; Reston, VA
- PMID
- 11438635
- ISSN
- 0161-5505
- eISSN
- 1535-5667
- Language
- English
- Date published
- 2001
- Academic Unit
- Pathology
- Record Identifier
- 9984047854402771
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