Journal article
Early ear neuronal development, but not olfactory or lens development, can proceed without SOX2
Developmental biology, Vol.457(1), pp.43-56
01/01/2020
DOI: 10.1016/j.ydbio.2019.09.003
PMID: 31526806
Abstract
SOX2 is essential for maintaining neurosensory stem cell properties, although its involvement in the early neurosensory development of cranial placodes remains unclear. To address this, we used Foxg1-Cre to conditionally delete Sox2 during eye, ear, and olfactory placode development. Foxg1-Cre mediated early deletion of Sox2 eradicates all olfactory placode development, and disrupts retinal development and invagination of the lens placode. In contrast to the lens and olfactory placodes, the ear placode invaginates and delaminates NEUROD1 positive neurons. Furthermore, we show that SOX2 is not necessary for early ear neurogenesis, since the early inner ear ganglion is formed with near normal central projections to the hindbrain and peripheral projections to the undifferentiated sensory epithelia of E11.5–12.5 ears. However, later stages of ear neurosensory development, in particular, the late forming auditory system, critically depend on the presence of SOX2. Our data establish distinct differences for SOX2 requirements among placodal sensory organs with similarities between olfactory and lens but not ear placode development, consistent with the unique neurosensory development and molecular properties of the ear.
•Foxg1-Cre-mediated Sox2 deletion disrupts olfactory and lens placode development.•Ear placode invagination and otocyst formation proceed without SOX2.•Early vestibular ganglion formation with neuron projections advances without SOX2.•Loss of SOX2 blocks cochlear neuronal and sensory development.•Overall ear morphogenesis is significantly reduced in the absence of SOX2.
Details
- Title: Subtitle
- Early ear neuronal development, but not olfactory or lens development, can proceed without SOX2
- Creators
- Martina Dvorakova - Institute of Biotechnology CAS, Vestec, CzechiaIva Macova - Institute of Biotechnology CAS, Vestec, CzechiaRomana Bohuslavova - Institute of Biotechnology CAS, Vestec, CzechiaMiroslava Anderova - Institute of Experimental Medicine CAS, Prague, CzechiaBernd Fritzsch - Department of Biology, University of Iowa, Iowa City, IA, USAGabriela Pavlinkova - Institute of Biotechnology CAS, Vestec, Czechia
- Resource Type
- Journal article
- Publication Details
- Developmental biology, Vol.457(1), pp.43-56
- DOI
- 10.1016/j.ydbio.2019.09.003
- PMID
- 31526806
- NLM abbreviation
- Dev Biol
- ISSN
- 0012-1606
- eISSN
- 1095-564X
- Publisher
- Elsevier Inc
- Grant note
- DOI: 10.13039/501100001823, name: MEYS CR, award: LM2015062; DOI: 10.13039/501100001824, name: Czech Science Foundation, award: 17-04719S; DOI: 10.13039/501100008530, name: European Regional Development Fund, award: CZ.1.05/1.1.00/02.0109; name: Institute of Biotechnology of the Czech Academy of Sciences, award: 86652036; DOI: 10.13039/100000002, name: NIH, award: R01 AG060504
- Language
- English
- Date published
- 01/01/2020
- Academic Unit
- Iowa Neuroscience Institute; Biology; Craniofacial Anomalies Research Center
- Record Identifier
- 9984070533002771
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