Early epithelial invasion by Salmonella enterica serovar Typhimurium DT104 in the swine ileum

D K Meyerholz; T J Stabel; M R Ackermann; S A Carlson; B D Jones; J Pohlenz

doi:10.1354/vp.39-6-712

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Early epithelial invasion by Salmonella enterica serovar Typhimurium DT104 in the swine ileum

Journal article

Peer reviewed

Early epithelial invasion by Salmonella enterica serovar Typhimurium DT104 in the swine ileum

D K Meyerholz, T J Stabel, M R Ackermann, S A Carlson, B D Jones and J Pohlenz

Veterinary pathology, Vol.39(6), pp.712-720

11/2002

DOI: 10.1354/vp.39-6-712

PMID: 12450202

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Abstract

Salmonella enterica serovar Typhimurium is an important intestinal pathogen in swine. This study was performed to document the early cellular invasion of Salmonella serovar Typhimurium in swine ileum. Ileal gut-loops were surgically prepared in ten 4- to 5-week-old mixed-breed pigs and inoculated for 0-60 minutes. Loops were harvested and prepared for both scanning and transmission electron microscopy (SEM and TEM, respectively). Preferential bacterial adherence to microfold cells (M cells) was seen within 5 minutes, and by 10 minutes bacterial invasion of the apical membrane was seen in M cells, goblet cells, and enterocytes. This multicellular invasion was observed throughout the course of infection. In addition, SEM revealed a specific affinity of Salmonella serovar Typhimurium to sites of cell extrusion. Using TEM, bacteria in these areas were focused in the crevices formed by the extruding cell and the adjacent cells and in the cytoplasm immediately beneath the extruding cell. Our results suggest that early cellular invasion by Salmonella serovar Typhimurium is nonspecific and rapid in swine. Furthermore, the combination of SEM and TEM data suggests that Salmonella serovar Typhimurium may use sites of cell extrusion as an additional mechanism for early invasion.

Ileum - pathology

Intestinal Mucosa - ultrastructure

Salmonella Infections, Animal - pathology

Salmonella typhimurium - growth & development

Swine Diseases - pathology

Intestinal Mucosa - microbiology

Random Allocation

Animals

Swine

Ileum - ultrastructure

Intestinal Diseases - microbiology

Salmonella Infections, Animal - microbiology

Swine Diseases - microbiology

Microscopy, Electron, Scanning - veterinary

In Vitro Techniques

Ileum - microbiology

Intestinal Diseases - veterinary

Intestinal Diseases - pathology

Intestinal Mucosa - pathology

Details

Title: Subtitle: Early epithelial invasion by Salmonella enterica serovar Typhimurium DT104 in the swine ileum
Creators: D K Meyerholz - Department of Veterinary Microbiology, Iowa State University, Ames, USA
T J Stabel
M R Ackermann
S A Carlson
B D Jones
J Pohlenz
Resource Type: Journal article
Publication Details: Veterinary pathology, Vol.39(6), pp.712-720
DOI: 10.1354/vp.39-6-712
PMID: 12450202
NLM abbreviation: Vet Pathol
ISSN: 0300-9858
eISSN: 1544-2217
Language: English
Date published: 11/2002
Academic Unit: Microbiology and Immunology; Pathology
Record Identifier: 9984083899702771

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