Early exposure to IL-4 stabilizes IL-4 mRNA in CD4+ T cells via RNA-binding protein HuR

Timur O Yarovinsky; Noah S Butler; Martha M Monick; Gary W Hunninghake

doi:10.4049/jimmunol.177.7.4426

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Early exposure to IL-4 stabilizes IL-4 mRNA in CD4+ T cells via RNA-binding protein HuR

Journal article

Peer reviewed

Early exposure to IL-4 stabilizes IL-4 mRNA in CD4+ T cells via RNA-binding protein HuR

Timur O Yarovinsky, Noah S Butler, Martha M Monick and Gary W Hunninghake

The Journal of immunology (1950), Vol.177(7), pp.4426-4435

10/01/2006

DOI: 10.4049/jimmunol.177.7.4426

PMID: 16982877

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Abstract

The mechanisms regulating IL-4 mRNA stability in differentiated T cells are not known. We found that early exposure of CD4+ T cells to endogenous IL-4 increased IL-4 mRNA stability. This effect of IL-4 was mediated by the RNA-binding protein HuR. IL-4 mRNA interacted with HuR and the dominant binding site was shown within the coding region of IL-4 mRNA. Exposure of CD4+ T cells to IL-4 had no effects on HuR expression or subcellular localization, but triggered HuR binding to IL-4 mRNA. Thus, IL-4 plays a positive role in maintaining IL-4 mRNA stability in CD4+ T cells via a HuR-mediated mechanism.

ELAV-Like Protein 1

RNA Stability - immunology

Gene Expression

Protein Structure, Secondary

Gene Expression Regulation - immunology

Interleukin-4 - metabolism

RNA, Messenger - genetics

CD4-Positive T-Lymphocytes - metabolism

Molecular Sequence Data

Immunoblotting

ELAV Proteins

CD4-Positive T-Lymphocytes - immunology

Microscopy, Confocal

Animals

Flow Cytometry

Base Sequence

Fluorescent Antibody Technique

Antigens, Surface - metabolism

RNA, Messenger - chemistry

Electrophoretic Mobility Shift Assay

Mice

Interleukin-4 - genetics

Lymphocyte Activation - physiology

RNA-Binding Proteins - metabolism

Details

Title: Subtitle: Early exposure to IL-4 stabilizes IL-4 mRNA in CD4+ T cells via RNA-binding protein HuR
Creators: Timur O Yarovinsky - Roy J. and Lucille A. Carver College of Medicine, University of Iowa and Veterans Administration Medical Center, Iowa City, IA 52242, USA. timur-yarovinsky@uiowa.edu
Noah S Butler
Martha M Monick
Gary W Hunninghake
Resource Type: Journal article
Publication Details: The Journal of immunology (1950), Vol.177(7), pp.4426-4435
DOI: 10.4049/jimmunol.177.7.4426
PMID: 16982877
NLM abbreviation: J Immunol
ISSN: 0022-1767
eISSN: 1550-6606
Publisher: United States
Grant note: HL 007638 / NHLBI NIH HHS HL 073967 / NHLBI NIH HHS HL 077431 / NHLBI NIH HHS RR 00059 / NCRR NIH HHS
Language: English
Date published: 10/01/2006
Academic Unit: Microbiology and Immunology; Internal Medicine
Record Identifier: 9984002377402771

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