Journal article
Echocardiographic Assessment of Cardiac Morphology and Function in Xenopus
Comparative medicine, Vol.60(2), pp.107-113
04/01/2010
PMCID: PMC2855036
PMID: 20412684
Abstract
Advances using
Xenopus
as a model permit valuable inquiries into cardiac development from embryo to adult. Noninvasive methods are needed to study cardiac function longitudinally. The objective of this study was to evaluate the feasibility of echocardiographic studies in
Xenopus
and establish normative data of adult cardiac structure and function. Doppler and 2D echocardiograms and electrocardiograms were acquired from adult
Xenopus laevis
and
X. tropicalis
. Frogs were exposed to either isoflurane or tricaine to discern the effect of sedating agents on cardiac function. Cardiac dimensions, morphology, flow velocities, and electrophysiologic intervals were measured and evaluated by using bivariate and regression analyses. Normal cardiac dimensions relative to body weight and species were established by echocardiography. Normal conduction intervals were determined by electrocardiography and did not vary by body weight or species. Anesthetic agent did not affect ejection fraction or flow velocity but did alter the QRS duration and QT interval. Echocardiographic and electrocardiographic studies in
Xenopus
provide information about cardiac anatomy and physiology and can readily be used for longitudinal analyses of developmental inquiries. Body weight, species, and anesthetic agent are factors that should be considered in experimental design and analyses.
Details
- Title: Subtitle
- Echocardiographic Assessment of Cardiac Morphology and Function in Xenopus
- Creators
- Heather L Bartlett - Department of Pediatrics, University of Iowa, Iowa City, IowaRobert B Escalera - Department of Pediatrics, University of Iowa, Iowa City, IowaSonali S Patel - University of IowaElesa W Wedemeyer - Department of Pediatrics, University of Iowa, Iowa City, IowaKenneth A Volk - Department of Pediatrics, University of Iowa, Iowa City, IowaJamie L Lohr - University of MinnesotaBenjamin E Reinking - University of Iowa
- Resource Type
- Journal article
- Publication Details
- Comparative medicine, Vol.60(2), pp.107-113
- Publisher
- American Association for Laboratory Animal Science
- PMID
- 20412684
- PMCID
- PMC2855036
- ISSN
- 1532-0820
- eISSN
- 2769-819X
- Language
- English
- Date published
- 04/01/2010
- Academic Unit
- Cardiology; Stead Family Department of Pediatrics
- Record Identifier
- 9984353949402771
Metrics
16 Record Views