Effect of 1–2 mmol/1 calcium, triamcinolone acetonide, and retinoids on low-calcium regulated keratinocyte differentiation

Cynthia L Marcelo; R C Gold; Janet A Fairley

doi:10.1111/j.1365-2133.1984.tb15583.x

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Effect of 1–2 mmol/1 calcium, triamcinolone acetonide, and retinoids on low-calcium regulated keratinocyte differentiation

Journal article

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Effect of 1–2 mmol/1 calcium, triamcinolone acetonide, and retinoids on low-calcium regulated keratinocyte differentiation

Cynthia L Marcelo, R C Gold and Janet A Fairley

British journal of dermatology (1951), Vol.111 Suppl 27(27), pp.64-72

07/1984

DOI: 10.1111/j.1365-2133.1984.tb15583.x

PMID: 6204678

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Abstract

Neonatal mouse keratinocytes cultured in low calcium (0.01 mmol/l) show rapid growth and little stratification when compared with cells grown in normal 1.2 mmol/l calcium. The effect of low calcium on the amount and synthesis of specific differentiation proteins was studied; additionally, the effect of 10(-8) mol/l triamcinolone acetonide, and 6 micrograms/ml of retinoic acid and of etretinate (Ro 10-9359) on low-calcium regulated keratinocyte hyperproliferation and differentiation was determined. Low-calcium regulated keratinocytes contained less non-covalently cross-linked and disulphide cross-linked keratins, less cell envelopes, much greater amounts of SDS-soluble viable cell proteins, and slightly more keratohyaline granule-related proteins than normal-calcium regulated keratinocytes. A 24 h switching time to 1.2 mmol/l calcium medium did not affect the amounts or synthesis of these proteins. Both retinoids and triamcinolone acetonide inhibited by approximately 50% the proliferation of the low-calcium regulated keratinocytes. Growth of low-calcium cells in these drugs for 9 days increased the amounts of both keratins and cell envelope proteins in the cultures. We concluded that calcium-dependent processes can regulate epidermal keratinocyte proliferation and differentiation. Our studies suggest that these calcium-regulated events may occur via changes in calcium-dependent proteins.

Leucine - metabolism

Protein Biosynthesis

Skin - cytology

Calcium - pharmacology

Skin - metabolism

Electrophoresis, Polyacrylamide Gel

Cells, Cultured

Triamcinolone Acetonide - pharmacology

Etretinate - pharmacology

Keratins - biosynthesis

Animals

Cell Differentiation - drug effects

DNA - biosynthesis

Mice

Skin - drug effects

Thymidine - metabolism

Tretinoin - pharmacology

Details

Title: Subtitle: Effect of 1–2 mmol/1 calcium, triamcinolone acetonide, and retinoids on low-calcium regulated keratinocyte differentiation
Creators: Cynthia L Marcelo
R C Gold
Janet A Fairley
Resource Type: Journal article
Publication Details: British journal of dermatology (1951), Vol.111 Suppl 27(27), pp.64-72
DOI: 10.1111/j.1365-2133.1984.tb15583.x
PMID: 6204678
NLM abbreviation: Br J Dermatol
ISSN: 0007-0963
eISSN: 1365-2133
Publisher: England
Grant note: AM 26009 / NIADDK NIH HHS
Language: English
Date published: 07/1984
Academic Unit: Dermatology
Record Identifier: 9984025673502771

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