Effect of Chitosan as a Cross-Linker on Matrix Metalloproteinase Activity and Bond Stability with Different Adhesive Systems

Eugenia Baena; Sandra R. Cunha; Tatjana Maravic; Allegra Comba; Federica Paganelli; Giulio Alessandri-Bonetti; Laura Ceballos; Franklin R. Tay; Lorenzo Breschi; Annalisa Mazzoni

doi:10.3390/md18050263

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Effect of Chitosan as a Cross-Linker on Matrix Metalloproteinase Activity and Bond Stability with Different Adhesive Systems

Journal article

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Effect of Chitosan as a Cross-Linker on Matrix Metalloproteinase Activity and Bond Stability with Different Adhesive Systems

Eugenia Baena, Sandra R. Cunha, Tatjana Maravic, Allegra Comba, Federica Paganelli, Giulio Alessandri-Bonetti, Laura Ceballos, Franklin R. Tay, Lorenzo Breschi and Annalisa Mazzoni

Marine drugs, Vol.18(5), p.263

05/18/2020

DOI: 10.3390/md18050263

PMCID: PMC7280998

PMID: 32443628

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Abstract

The aim of the present study was to evaluate the effect of 0.1% chitosan (Ch) solution as an additional primer on the mechanical durability and enzymatic activity on dentine using an etch-and-rinse (E&R) adhesive and a universal self-etch (SE) adhesive. Microtensile bond strength and interfacial nanoleakage expression of the bonded interfaces for all adhesives (with or without pretreatment with 0.1% Ch solution for 1 min and air-dried for 5 s) were analyzed immediately and after 10,000 thermocycles. Zymograms of protein extracts from human dentine powder incubated with Optibond FL and Scotchbond Universal on untreated or Ch-treated dentine were obtained to examine dentine matrix metalloproteinase (MMP) activities. The use of 0.1% Ch solution as an additional primer in conjunction with the E&R or SE adhesive did not appear to have influenced the immediate bond strength (T0) or bond strength after thermocycling (T1). Zymography showed a reduction in MMP activities only for mineralized and demineralized dentine powder after the application of Ch. Application of 0.1% Ch solution does not increase the longevity of resin-dentine bonds. Nonetheless, the procedure appears to be proficient in reducing dentine MMP activities within groups without adhesive treatments. Further studies are required to comprehend the cross-linking of Ch with dentine collagen.

Chemistry, Medicinal

Life Sciences & Biomedicine

Pharmacology & Pharmacy

Science & Technology

Details

Title: Subtitle: Effect of Chitosan as a Cross-Linker on Matrix Metalloproteinase Activity and Bond Stability with Different Adhesive Systems
Creators: Eugenia Baena - Universidad Rey Juan Carlos
Sandra R. Cunha - University of Iowa
Tatjana Maravic - University of Novi Sad
Allegra Comba - University of Bologna
Federica Paganelli - University of Bologna
Giulio Alessandri-Bonetti - University of Bologna
Laura Ceballos - Universidad Rey Juan Carlos
Franklin R. Tay - Augusta University
Lorenzo Breschi - University of Bologna
Annalisa Mazzoni - University of Bologna
Resource Type: Journal article
Publication Details: Marine drugs, Vol.18(5), p.263
Publisher: Mdpi
DOI: 10.3390/md18050263
PMID: 32443628
PMCID: PMC7280998
ISSN: 1660-3397
eISSN: 1660-3397
Number of pages: 14
Grant note: European Federation of Conservative Dentistry
Language: English
Date published: 05/18/2020
Academic Unit: Operative Dentistry
Record Identifier: 9984696655302771

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