Journal article
Effect of Deep Tissue Incision on pH Responses of Afferent Fibers and Dorsal Root Ganglia Innervating Muscle
Anesthesiology (Philadelphia), Vol.119(5), pp.1186-1197
11/01/2013
DOI: 10.1097/ALN.0b013e31829bd791
PMCID: PMC4028173
PMID: 23732174
Abstract
Background: Understanding the mechanisms underlying deep tissue pain in the postoperative period is critical to improve therapies. Using the in vitro plantar flexor digitorum brevis muscle-nerve preparation and patch clamp recordings from cultured dorsal root ganglia neurons innervating incised and unincised muscle, the authors investigated responses to various pH changes.
Methods: Incision including the plantar flexor digitorum brevis muscle or sham operation was made in the rat hind paw. On postoperative day 1, in vitro single-fiber recording was undertaken. On the basis of previous studies, the authors recorded from at least 40 fibers per group. Also DiI-labeled dorsal root ganglia innervating muscle from rats undergoing incision and a sham operation were cultured and tested for acid responses, using whole cell patch clamp recordings.
Results: The prevalence of responsive group IV afferents to lactic acid pH 6.5 in the incision group (15 of 67; 22.3%) was greater than that in the control group (2 of 35; 5.7%; P = 0.022). In dorsal root ganglia neurons innervating muscle, incision increased mean current amplitudes of acid-evoked currents; the acid-sensing ion channel blocker, amiloride 300 m, inhibited more than 75% of the acid-evoked current, whereas, the transient receptor vanilloid receptor 1 blocker (AMG9810 1 M) did not cause significant inhibition.
Conclusion: The authors' experiments demonstrated that incision increases the responses of flexor digitorum brevis muscle afferent fibers to weak acid solutions, and increased acid-evoked currents in dorsal root ganglia innervating muscle. The authors' data suggest that up-regulation of acid-sensing ion channels might underlie this increased chemosensitivity caused by surgery.
Details
- Title: Subtitle
- Effect of Deep Tissue Incision on pH Responses of Afferent Fibers and Dorsal Root Ganglia Innervating Muscle
- Creators
- Kanta Kido - University of Iowa Hospitals and ClinicsMamta Gautam - Research Associate, Department of Internal MedicineChristopher J. Benson - Roy J. and Lucille A. Carver College of MedicineHe Gu - University of Iowa Hospitals and ClinicsTimothy J. Brennan - University of Iowa Hospitals and Clinics
- Resource Type
- Journal article
- Publication Details
- Anesthesiology (Philadelphia), Vol.119(5), pp.1186-1197
- DOI
- 10.1097/ALN.0b013e31829bd791
- PMID
- 23732174
- PMCID
- PMC4028173
- NLM abbreviation
- Anesthesiology
- ISSN
- 0003-3022
- eISSN
- 1528-1175
- Publisher
- Lippincott Williams & Wilkins
- Number of pages
- 12
- Grant note
- GM067762 / National Institutes of Health, Bethesda, Maryland; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA R01GM067762 / NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Institute of General Medical Sciences (NIGMS)
- Language
- English
- Date published
- 11/01/2013
- Academic Unit
- Cardiovascular Medicine; Anesthesia; Fraternal Order of Eagles Diabetes Research Center; Neuroscience and Pharmacology; Internal Medicine
- Record Identifier
- 9984303747502771
Metrics
9 Record Views