Journal article
Effect of Free Radical Scavengers on Changes in Ion Conductance During Exposure to Therapeutic Ultrasound
Molecular membrane biology, Vol.10(4), pp.237-247
1993
DOI: 10.3109/09687689309150271
PMID: 8007841
Abstract
Ultrasound has been used in physical therapy for > 4 decades. Recent studies indicate that non-thermal mechanisms such as cavitation are involved in the observed effects. Free radicals and other highly reactive compounds are known to form during sonochemical reactions associated with acoustic cavitation. Using frog skin as a biological model, the possibility that the increase in ionic conductance (Gt) upon exposure to therapeutic ultrasound is due to the effect of free radicals generated by sonochemical reactions, was investigated. It was found that the presence of cystamine, cysteamine and sodium ascorbate significantly reduced the increase in conductance caused by the exposure to 300 mW/cm2 (1 MHz CW) therapeutic ultrasound. The attentuation in the effects was dependent on the concentration of the radical scavengers/antioxidants used, the incubation time, and the intensity of ultrasound. The effects were also dependent on the lipid solubility of free radical scavengers/antioxidants. The time constant for the recovery process of Gt in the presence of free radical scavengers and antioxidants after exposure to ultrasound was found to be not significantly different from control. These results suggest that the increase in Gt due to ultrasound is induced by free radicals and other reactive species generated from acoustic cavitation. This study provides an indirect evidence to the contingent that free radicals are generated and act inside the cells. Furthermore, the radical scavengers and antioxidants used provide protection from oxidative damage without being involved in the recovery of Gt towards steady state values after sonication.
Details
- Title: Subtitle
- Effect of Free Radical Scavengers on Changes in Ion Conductance During Exposure to Therapeutic Ultrasound
- Creators
- M. A Adinno - 1Department of Physics, East Carolina University, Greenville, NC, 27858A. M Al-karmi - 1Department of Physics, East Carolina University, Greenville, NC, 27858D. A Stoltz - 2Department of Physics and Astronomy, University of Mississippi, University, MS, 38677J. C Matthews - 3Department of Pharmacology, University of Mississippi, University, MS, 38677L. A Crum - 4Applied Physics Laboratory, University of Washington, Seattle, WA, 98105
- Resource Type
- Journal article
- Publication Details
- Molecular membrane biology, Vol.10(4), pp.237-247
- Publisher
- Informa UK Ltd
- DOI
- 10.3109/09687689309150271
- PMID
- 8007841
- ISSN
- 0968-7688
- eISSN
- 1464-5203
- Language
- English
- Date published
- 1993
- Academic Unit
- Roy J. Carver Department of Biomedical Engineering; Molecular Physiology and Biophysics; Pulmonary, Critical Care, and Occupational Medicine; Internal Medicine
- Record Identifier
- 9984025440602771
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