Journal article
Effect of Ischemia Duration on Autoantibody Response in Rats Undergoing Retinal Ischemia-Reperfusion
Ophthalmic research, Vol.48(2), pp.67-74
07/2012
DOI: 10.1159/000335965
PMID: 22472973
Abstract
Both the innate and the adaptive immune systems are involved in the pathogenic processes following ischemia-reperfusion injury. We analyzed the possible correlation between the duration of ischemia and autoantibody diversification in a model of ocular ischemia. Rats were subjected to 30, 45, or 90 min of ischemia, and retinal ganglion cell (RGC) density and antibody reactivity were analyzed via customized protein microarray slides. After ocular ischemia, significant alterations in antibody response were observed, while increasing exposure caused more severe RGC damage. Distinct antibody responses after ischemia were detected; these alterations comprised decreased reactivities against cyclophilin A and glyceraldehyde-3-phosphate dehydrogenase, possibly due to increased binding of circulating antibodies to debris material. Other antibodies, like those against α5β1-integrin or β2-adrenergic receptor, were upregulated after ischemia.
Details
- Title: Subtitle
- Effect of Ischemia Duration on Autoantibody Response in Rats Undergoing Retinal Ischemia-Reperfusion
- Creators
- Stephanie C JoachimThomas JehleNils BoehmOliver W GramlichWolf A LagrezeNorbert PfeifferFranz H Grus
- Resource Type
- Journal article
- Publication Details
- Ophthalmic research, Vol.48(2), pp.67-74
- Publisher
- Basel, Switzerland
- DOI
- 10.1159/000335965
- PMID
- 22472973
- ISSN
- 0030-3747
- eISSN
- 1423-0259
- Number of pages
- 8
- Language
- English
- Date published
- 07/2012
- Academic Unit
- Iowa Neuroscience Institute; Neuroscience and Pharmacology; Ophthalmology and Visual Sciences
- Record Identifier
- 9984070454202771
Metrics
10 Record Views