Journal article
Effect of L-fucose on proliferation and myo-inositol metabolism in cultured cerebral microvessel and aortic endothelial cells
Journal of cellular physiology, Vol.153(2), pp.321-331
11/1992
DOI: 10.1002/jcp.1041530212
PMID: 1429852
Abstract
Decreased myo-inositol metabolism possibly contributes to the development of diabetic complications including micro and macrovascular disease. Previous studies have shown that hyperglycemia may be partially responsible for this defect. We have found that L-fucose, a monosaccharide present in low concentrations in normal circulation and found to be elevated in diabetes, causes defects in cultured endothelial cells, including alterations in myo-inositol metabolism and proliferation. Murine cerebral microvessel and bovine aortic endothelial cells take up L-fucose from the medium in a time and concentration-dependent manner. Both acute and chronic exposure of these cultured endothelial cells to media containing L-fucose at concentrations that may exist in diabetic sera cause a significant decrease in the accumulation of myo-inositol and its incorporation into inositol phospholipids. There is a concomitant decrease in the intracellular levels of myo-inositol. Kinetic analysis of the effect of L-fucose on myo-inositol uptake suggests that L-fucose competitively inhibits the transport of myo-inositol, exhibiting a Ki in the range of 1.6-4.1 mM for both cell types. Endothelial cells exposed to L-fucose concentrations of 0.5-20 mM exhibit depressed rates of proliferation in a concentration-dependent fashion. Furthermore, L-fucose causes a concentration-dependent decrease in synthesis of proteoglycan by cultured cerebral microvessel endothelial cells as measured by incorporation of 35S; however, this effect is not observed in the aortic endothelia. These data suggest that L-fucose at concentrations that may exist in diabetic sera may impair myo-inositol metabolism and proliferation of the vascular endothelium.
Details
- Title: Subtitle
- Effect of L-fucose on proliferation and myo-inositol metabolism in cultured cerebral microvessel and aortic endothelial cells
- Creators
- Mark R Stefani - Department of Internal Medicine, Veterans Administration Medical Center and Diabetes Endocrinology Research Center, University of Iowa, Iowa City, Iowa 52242Joyce A Dunlap - Department of Internal Medicine, Veterans Administration Medical Center and Diabetes Endocrinology Research Center, University of Iowa, Iowa City, Iowa 52242Mark A Yorek - Department of Internal Medicine, Veterans Administration Medical Center and Diabetes Endocrinology Research Center, University of Iowa, Iowa City, Iowa 52242
- Resource Type
- Journal article
- Publication Details
- Journal of cellular physiology, Vol.153(2), pp.321-331
- Publisher
- Wiley Subscription Services, Inc., A Wiley Company
- DOI
- 10.1002/jcp.1041530212
- PMID
- 1429852
- ISSN
- 0021-9541
- eISSN
- 1097-4652
- Number of pages
- 11
- Language
- English
- Date published
- 11/1992
- Academic Unit
- Fraternal Order of Eagles Diabetes Research Center; Endocrinology and Metabolism; Internal Medicine
- Record Identifier
- 9984094641802771
Metrics
12 Record Views