Effect of Merocel vaginal sponge on growth of Staphylococcus aureus and production of toxic shock syndrome-associated toxins

P M Schlievert

Back

Journal article

Effect of Merocel vaginal sponge on growth of Staphylococcus aureus and production of toxic shock syndrome-associated toxins

P M Schlievert

Journal of the American College of Surgeons, Vol.183(1), pp.19-24

07/1996

PMID: 8673303

View Online

Abstract

Staphylococcus aureus toxic shock syndrome toxin-1 and enterotoxin B are the major causes of toxic shock syndrome. These toxins are produced in sufficient concentrations to produce illness in the presence of certain tampons. This necessitates evaluating tampons, as well as wound dressings for their effects on S. aureus growth and toxin production. In this study, the Merocel vaginal sponge was evaluated both in vitro and in vivo in a rabbit model for effect on S. aureus. The Merocel sponge was tested in Erlenmeyer shake flasks containing growth media and in dialysis tubing immersed in agar growth media for both effect on S. aureus plate counts compared to media alone and effect on production of toxic shock syndrome toxins. The in vivo test included placement of Merocel sponges subcutaneously along the flanks of rabbits with subsequent inoculation with toxic shock syndrome bacteria and evaluation for development of illness and death. In the two standard in vitro tests, the shake flask and tampon sac, the Merocel sponge inhibited both growth of toxic shock syndrome S. aureus and production of toxic shock syndrome toxin-1 and enterotoxin B. The Merocel sponge also prevented development of toxic shock syndrome in a rabbit model. The data suggest the Merocel sponge may reduce the risk of development of toxic shock syndrome in association with its use. These studies may serve as models for evaluation of other products that are intended to be used on mucosal and skin surfaces, for their effect on toxic shock syndrome toxins.

Rabbits

Shock, Septic - etiology

Polyvinyl Alcohol - pharmacology

Formaldehyde - pharmacology

Hemostatics - pharmacology

Shock, Septic - prevention & control

Tampons, Surgical

Enterotoxins - biosynthesis

Animals

Bacterial Toxins

Culture Media

Superantigens

Enterotoxins - antagonists & inhibitors

Female

Drug Evaluation, Preclinical

Staphylococcus aureus - drug effects

Staphylococcus aureus - growth & development

Staphylococcus aureus - metabolism

Details

Title: Subtitle: Effect of Merocel vaginal sponge on growth of Staphylococcus aureus and production of toxic shock syndrome-associated toxins
Creators: P M Schlievert - Department of Microbiology, University of Minnesota Medical School, Minneapolis 55455, USA
Resource Type: Journal article
Publication Details: Journal of the American College of Surgeons, Vol.183(1), pp.19-24
Publisher: United States
PMID: 8673303
ISSN: 1072-7515
eISSN: 1879-1190
Grant note: AI22159 / NIAID NIH HHS
Language: English
Date published: 07/1996
Academic Unit: Microbiology and Immunology; Internal Medicine
Record Identifier: 9984002388202771

Metrics

11 Record Views

8 Times Cited - Web of Science