Journal article
Effect of Nitrogen Dioxide Exposure on Allergic Asthma in a Murine Model
Chest, Vol.126(1), pp.198-204
07/2004
DOI: 10.1378/chest.126.1.198
PMID: 15249463
Abstract
The purpose of this study was to examine the effects of NO2, a major component of air pollution, on airway eosinophilic inflammation and bronchial hyperreactivity, using a mouse model of asthma.
BALB/c mice (eight mice per experimental group) were studied in a basic research laboratory at the University of Iowa.
Using a standard murine model of asthma, BALB/c mice were sensitized to ovalbumin (OVA) by intraperitoneal (IP) injections (days 1 and 7) and were challenged with aerosolized OVA (days 13 and 14). Some mice were exposed to NO2 (2 ppm) in an exposure chamber for 24 h before undergoing OVA aerosol challenge. A control group was exposed to OVA alone.
The outcomes assessed included airway inflammation, bronchial hyperreactivity to inhaled methacholine, and goblet cell hyperplasia. We found that NO2 exposure modestly increased airway neutrophilia but not airway eosinophilia in OVA-exposed mice. These mice exhibited epithelial damage and loss of epithelial mucin. Surprisingly, nonspecific bronchial hyperreactivity (ie, enhanced pause index) was not increased, although baseline smooth muscle tone was increased (p < 0.05) in the mice exposed to NO2.
These data indicate that relatively short-term (24 h) exposure to NO2 causes epithelial damage, reduced mucin expression, and increased tone of respiratory smooth muscle. Reduced mucin production may be a mechanism of injury following long-term exposure to inhaled NO2. Despite enhancing epithelial damage in OVA-exposed mice, NO2 exposure does not otherwise alter the expression of allergen-induced airway responses.
Details
- Title: Subtitle
- Effect of Nitrogen Dioxide Exposure on Allergic Asthma in a Murine Model
- Creators
- Iftikhar Hussain - Department of Medicine, Washington University School of Medicine, St. Louis, MOVipul V Jain - Department of Medicine, University of California San Francisco-Fresno, Fresno, CAPatrick O’Shaughnessy - Department of Occupational and Environmental Medicine, College of Public Health, University of Iowa, Iowa City, IAThomas R Businga - Division of Pulmonary Medicine, Department of Internal Medicine, Roy J. and Lucille A. Carver College of Medicine, Iowa City, IAJoel Kline - Division of Pulmonary Medicine, Department of Internal Medicine, Roy J. and Lucille A. Carver College of Medicine, Iowa City, IA
- Resource Type
- Journal article
- Publication Details
- Chest, Vol.126(1), pp.198-204
- Publisher
- Elsevier Inc
- DOI
- 10.1378/chest.126.1.198
- PMID
- 15249463
- ISSN
- 0012-3692
- eISSN
- 1931-3543
- Language
- English
- Date published
- 07/2004
- Academic Unit
- Pulmonary, Critical Care, and Occupational Medicine; Civil and Environmental Engineering; Occupational and Environmental Health; Pathology; Internal Medicine
- Record Identifier
- 9984094760102771
Metrics
20 Record Views