Journal article
Effect of Primer Selection on Estimates of GB Virus C (GBV-C) Prevalence and Response to Antiretroviral Therapy for Optimal Testing for GBV-C Viremia
Journal of clinical microbiology, Vol.44(9), pp.3105-3113
09/2006
DOI: 10.1128/JCM.02663-05
PMCID: PMC1594694
PMID: 16954234
Abstract
GB virus C (GBV-C; also called hepatitis G virus) is a common cause of infection associated with prolonged survival among HIV-infected individuals. The prevalences of GBV-C viremia vary widely in different studies, and there has been poor agreement among different laboratories performing GBV-C RNA detection in quality control studies. To determine the optimal method of measuring GBV-C RNA in clinical samples, samples obtained from 939 HIV-infected subjects were studied using reverse transcription (RT)-PCR methods amplifying four separate regions of the GBV-C genome. Primers amplifying the E2 coding region were 100% specific; however, their sensitivity was only 76.6%. In contrast, primers amplifying three additional conserved regions of the GBV-C genome (the 5′ nontranslated region and the nonstructural protein-coding regions 3 and 5A) were more sensitive but produced higher rates of false-positive results. Using low-specificity primer sets influenced the significance of association between GBV-C viremia and response to antiretroviral therapy. Using a quantitative GBV-C RNA method, the GBV-C RNA concentration did not correlate with baseline or set point HIV RNA levels; however, a correlation between negative, low, and high GBV-C RNA levels and increasing reduction in HIV RNA following antiretroviral therapy was observed. Subjects with both GBV-C E2 antibody and viremia had significantly lower GBV-C RNA levels than did viremic subjects without E2 antibody. These studies demonstrate that accurate detection of GBV-C RNA by nested RT-PCR requires the use of primers representing multiple genome regions. Analyses based on testing with single primers do not lead to reliable conclusions about the association between GBV-C infection and clinical outcomes.
Details
- Title: Subtitle
- Effect of Primer Selection on Estimates of GB Virus C (GBV-C) Prevalence and Response to Antiretroviral Therapy for Optimal Testing for GBV-C Viremia
- Creators
- I. E Souza - Department of Internal Medicine, University of Iowa Roy and Lucille Carver College of Medicine, and Iowa City Veterans Administration Medical Center, Iowa City, IowaJ. B Allen - Department of Internal Medicine, University of Iowa Roy and Lucille Carver College of Medicine, and Iowa City Veterans Administration Medical Center, Iowa City, IowaJ Xiang - Department of Internal Medicine, University of Iowa Roy and Lucille Carver College of Medicine, and Iowa City Veterans Administration Medical Center, Iowa City, IowaD Klinzman - Department of Internal Medicine, University of Iowa Roy and Lucille Carver College of Medicine, and Iowa City Veterans Administration Medical Center, Iowa City, IowaR Diaz - Department of Internal Medicine, University of Iowa Roy and Lucille Carver College of Medicine, and Iowa City Veterans Administration Medical Center, Iowa City, IowaS Zhang - Department of Internal Medicine, University of Iowa Roy and Lucille Carver College of Medicine, and Iowa City Veterans Administration Medical Center, Iowa City, IowaK Chaloner - Department of Internal Medicine, University of Iowa Roy and Lucille Carver College of Medicine, and Iowa City Veterans Administration Medical Center, Iowa City, IowaD Zdunek - Department of Internal Medicine, University of Iowa Roy and Lucille Carver College of Medicine, and Iowa City Veterans Administration Medical Center, Iowa City, IowaG Hess - Department of Internal Medicine, University of Iowa Roy and Lucille Carver College of Medicine, and Iowa City Veterans Administration Medical Center, Iowa City, IowaC. F Williams - Department of Internal Medicine, University of Iowa Roy and Lucille Carver College of Medicine, and Iowa City Veterans Administration Medical Center, Iowa City, IowaL Benning - Department of Internal Medicine, University of Iowa Roy and Lucille Carver College of Medicine, and Iowa City Veterans Administration Medical Center, Iowa City, IowaJ. T Stapleton - Department of Internal Medicine, University of Iowa Roy and Lucille Carver College of Medicine, and Iowa City Veterans Administration Medical Center, Iowa City, Iowa
- Resource Type
- Journal article
- Publication Details
- Journal of clinical microbiology, Vol.44(9), pp.3105-3113
- DOI
- 10.1128/JCM.02663-05
- PMID
- 16954234
- PMCID
- PMC1594694
- NLM abbreviation
- J Clin Microbiol
- ISSN
- 0095-1137
- eISSN
- 1098-660X
- Publisher
- American Society for Microbiology
- Language
- English
- Date published
- 09/2006
- Academic Unit
- Microbiology and Immunology; Infectious Diseases; Internal Medicine
- Record Identifier
- 9984094643002771
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