Journal article
Effect of a Low-Fat Fish Oil Diet on Proinflammatory Eicosanoids and Cell-Cycle Progression Score in Men Undergoing Radical Prostatectomy
Cancer prevention research (Philadelphia, Pa.), Vol.7(1), pp.97-104
01/01/2014
DOI: 10.1158/1940-6207.CAPR-13-0261
PMID: 24169960
Abstract
We previously reported that a 4- to 6-week low-fat fish oil (LFFO) diet did not affect serum insulin-like growth factor (IGF)-1 levels (primary outcome) but resulted in lower omega-6 to omega-3 fatty acid ratios in prostate tissue and lower prostate cancer proliferation (Ki67) as compared with a Western diet. In this post hoc analysis, the effect of the LFFO intervention on serum pro-inflammatory eicosanoids, leukotriene B4 (LTB4) and 15-S-hydroxyeicosatetraenoic acid [15(S)-HETE], and the cell-cycle progression (CCP) score were investigated. Serum fatty acids and eicosanoids were measured by gas chromatography and ELISA. CCP score was determined by quantitative real-time reverse transcriptase PCR (RT-PCR). Associations between serum eicosanoids, Ki67, and CCP score were evaluated using partial correlation analyses. BLT1 (LTB4 receptor) expression was determined in prostate cancer cell lines and prostatectomy specimens. Serum omega-6 fatty acids and 15(S)-HETE levels were significantly reduced, and serum omega-3 levels were increased in the LFFO group relative to the Western diet group, whereas there was no change in LTB4 levels. The CCP score was significantly lower in the LFFO compared with the Western diet group. The 15(S)-HETE change correlated with tissue Ki67 (R = 0.48; P < 0.01) but not with CCP score. The LTB4 change correlated with the CCP score (r = 0.4; P = 0.02) but not with Ki67. The LTB4 receptor BLT1 was detected in prostate cancer cell lines and human prostate cancer specimens. In conclusion, an LFFO diet resulted in decreased 15 (S)-HETE levels and lower CCP score relative to a Western diet. Further studies are warranted to determine whether the LFFO diet antiproliferative effects are mediated through the LTB4/BLT1 and 15(S)-HETE pathways.
Details
- Title: Subtitle
- Effect of a Low-Fat Fish Oil Diet on Proinflammatory Eicosanoids and Cell-Cycle Progression Score in Men Undergoing Radical Prostatectomy
- Creators
- Colette Galet - University of California, Los AngelesKiran Gollapudi - University of Southern CaliforniaSevan Stepanian - Center for Human GeneticsJoshua B. Byrd - University of Southern CaliforniaSusanne M. Henning - University of California, Los AngelesTristan Grogan - Center for Human GeneticsDavid Elashoff - University of California, Los AngelesDavid Heber - University of Southern CaliforniaJonathan Said - University of California, Los AngelesPinchas Cohen - University of California, Los AngelesWilliam J. Aronson - Center for Human Genetics
- Resource Type
- Journal article
- Publication Details
- Cancer prevention research (Philadelphia, Pa.), Vol.7(1), pp.97-104
- DOI
- 10.1158/1940-6207.CAPR-13-0261
- PMID
- 24169960
- NLM abbreviation
- Cancer Prev Res (Phila)
- ISSN
- 1940-6207
- eISSN
- 1940-6215
- Publisher
- Amer Assoc Cancer Research
- Number of pages
- 8
- Grant note
- NIH P30CA016042 / UCLA Center UL1TR000124 / NATIONAL CENTER FOR ADVANCING TRANSLATIONAL SCIENCES; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Center for Advancing Translational Sciences (NCATS) P50CA92131 / NIH; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA UL1TR000124 / CTSI NIH P50CA092131 / NATIONAL CANCER INSTITUTE; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Cancer Institute (NCI)
- Language
- English
- Date published
- 01/01/2014
- Academic Unit
- Injury Prevention Research Center; University of Iowa Health Care
- Record Identifier
- 9985137927202771
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