Journal article
Effect of a modulator deletion on transcription of the human cytomegalovirus major immediate-early genes in infected undifferentiated and differentiated cells
Journal of virology, Vol.71(2), pp.1246-1255
02/1997
DOI: 10.1128/jvi.71.2.1246-1255.1997
PMCID: PMC191179
PMID: 8995648
Abstract
Differentiation-dependent expression of the human cytomegalovirus (HCMV) major immediate-early (MIE) genes, encoding IE1 and IE2, may partly govern virus replication in monocytic THP-1 and embryonal carcinoma (Tera-2) cells. The modulator of the MIE promoter was shown previously in transient transfection assays to repress transcription from promoter segments in undifferentiated THP-1 and Tera-2 cells but not in differentiated cells. To determine the biological importance of these findings, we constructed a recombinant HCMV (r delta MSVgpt) without a modulator. In comparison to wild-type (WT) virus, r delta MSVgpt exhibits a slight delay in growth in human fibroblasts, but there is no appreciable change in IE1 and IE2 transcription. Moreover, there is no appreciable change in the early/late kinetics of transcription of RNAs colinear with the predicted UL128 coding region, which is adjacent to the modulator, although the size distribution and abundance of these RNAs are altered. In infected undifferentiated THP-1 and Tera-2 cells, WT and r alpha MSVgpt viruses produce minimal but comparable amounts of IE1 RNAs. The genomes of both viruses are detectable in similar amounts within these undifferentiated cells. Induction of cellular differentiation before infection overcomes the block in MIE gene transcription. WT and r alpha MSVgpt infections of differentiated THP-1 cells produce similar levels of IE1 and IE2 RNAs. Thus, differentiation-dependent control of MIE gene transcription involves regulatory mechanisms other than the modulator. Possible alternative functions of the modulator are discussed.
Details
- Title: Subtitle
- Effect of a modulator deletion on transcription of the human cytomegalovirus major immediate-early genes in infected undifferentiated and differentiated cells
- Creators
- J L Meier - Department of Internal Medicine, University of Iowa College of Medicine, Iowa City 52242, USAM F Stinski
- Resource Type
- Journal article
- Publication Details
- Journal of virology, Vol.71(2), pp.1246-1255
- DOI
- 10.1128/jvi.71.2.1246-1255.1997
- PMID
- 8995648
- PMCID
- PMC191179
- ISSN
- 0022-538X
- eISSN
- 1098-5514
- Grant note
- HL37121 / NHLBI NIH HHS AI-13562 / NIAID NIH HHS
- Language
- English
- Date published
- 02/1997
- Academic Unit
- Microbiology and Immunology; Infectious Diseases; Epidemiology; Internal Medicine
- Record Identifier
- 9984094675302771
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