Effect of ammonium formate washing on the elemental composition determination in Nannochloropsis oceanica

Bárbara O. Guimarães; Pieter Gremmen; René H. Wijffels; Maria J. Barbosa; Sarah D'Adamo

doi:10.1016/j.aquaculture.2021.736526

Back

Effect of ammonium formate washing on the elemental composition determination in Nannochloropsis oceanica

Journal article

Open access

Peer reviewed

Effect of ammonium formate washing on the elemental composition determination in Nannochloropsis oceanica

Bárbara O. Guimarães, Pieter Gremmen, René H. Wijffels, Maria J. Barbosa and Sarah D'Adamo

Aquaculture, Vol.538, p.736526

05/30/2021

DOI: 10.1016/j.aquaculture.2021.736526

Files and links (1)

url

https://doi.org/10.1016/j.aquaculture.2021.736526View

Published (Version of record) Open Access

Abstract

This study investigated the effect of salt presence and removal on the integrity and elemental determination of the marine microalgae Nannochloropsis oceanica. Cells were harvested by centrifugation and washed with ammonium formate five times and subjected to mineral analysis after each washing step. Supernatants from each washing step were also analysed. Ca, Cu, Fe, Mg, Mn, Na, P, K, S, Zn were determined by inductively coupled plasma – optical emission spectrometry (ICP-OES). Results showed that washing microalgal biomass twice is sufficient to eliminate equipment oversaturation, allowing for more accurate elemental analysis, by using matrix matching. Moreover, washing the biomass did not rupture the cells, removed cell culture debris and decreased the concentration of Na, K, Ca present in the leftover growth medium. This study provides a reliable protocol for elemental determination in marine microalgae saving time in sample processing and analysis. [Display omitted] •Unwashed microalgal biomass samples lead to oversaturation of the ICP-OES machine.•Matrix matching is an effective tool when working with pre-washed microalgal biomass.•Matrix interference can be removed by the use of an internal standard or by dilution.•Washing biomass samples twice is sufficient to remove debris and salt in excess.

Ammonium formate

Elemental composition

Inductively coupled plasma - optical emission spectroscopy (ICP-OES)

Minerals in microalgae

Sodium

Details

Title: Subtitle: Effect of ammonium formate washing on the elemental composition determination in Nannochloropsis oceanica
Creators: Bárbara O. Guimarães - Wageningen University & Research
Pieter Gremmen - Wageningen University & Research
René H. Wijffels - Nord University
Maria J. Barbosa - Wageningen University and Research (WUR), Bioprocess Engineering, AlgaePARC, P.O. Box 16, 6700, AA, Wageningen, Netherlands
Sarah D'Adamo - Wageningen University & Research
Resource Type: Journal article
Publication Details: Aquaculture, Vol.538, p.736526
DOI: 10.1016/j.aquaculture.2021.736526
ISSN: 0044-8486
eISSN: 1873-5622
Publisher: Elsevier B.V
Grant note: DOI: 10.13039/501100000780, name: European Commission
Language: English
Date published: 05/30/2021
Academic Unit: International Programs; Spanish and Portuguese
Record Identifier: 9984398011202771

Metrics

21 Record Views

11 Times Cited - Web of Science