Journal article
Effect of anesthesia on renal R2 measured by blood oxygen level-dependent MRI
NMR in biomedicine, Vol.28(7), pp.811-817
07/2015
DOI: 10.1002/nbm.3314
PMCID: PMC4478156
PMID: 25951135
Abstract
Blood oxygen level-dependent (BOLD) MRI is increasingly being used to assess renal tissue oxygenation during disease based on the transverse relaxation rate (R2 *). In preclinical small animal models, the requisite use of anesthesia during imaging may lead to functional changes which influence R2 * and confound results. The purpose of this study was to evaluate the effects of four common anesthetic compounds on renal R2 * in healthy mice. Five female ICR mice were imaged with BOLD MRI approximately 25 min after induction with isoflurane (Iso; 1% or 1.5%, delivered in 100% O2 ), ketamine/xylazine (KX), sodium pentobarbital (PB) or 2,2,2-tribromoethanol (TBE). A significant effect of anesthetic agent on R2 * was observed in all tissue layers of the kidney, including the cortex, outer stripe of the outer medulla (OSOM), inner stripe of the outer medulla (ISOM) and inner medulla (IM). Pairwise significant differences in R2 * between specific agents were found in the cortex, OSOM and ISOM, with the largest difference observed in the ISOM between 1.5% Iso (26.6 ± 1.7 s(-1) ) and KX (66.0 ± 7.1 s(-1) ). The difference between 1% Iso and KX in the ISOM was not abolished when KX was administered with supplemental 100% O2 or when 1% Iso was delivered in 21% O2 , indicating that the fraction of inspired oxygen did not account for the observed differences. Our results indicate that the choice of anesthesia has a large influence on the observed R2 * in mouse kidney, and anesthetic effects must be considered in the design and interpretation of renal BOLD MRI studies.
Details
- Title: Subtitle
- Effect of anesthesia on renal R2 measured by blood oxygen level-dependent MRI
- Creators
- David J Niles - University of Wisconsin–MadisonJeremy W Gordon - University of Wisconsin–MadisonSean B Fain - University of Wisconsin–Madison
- Resource Type
- Journal article
- Publication Details
- NMR in biomedicine, Vol.28(7), pp.811-817
- DOI
- 10.1002/nbm.3314
- PMID
- 25951135
- PMCID
- PMC4478156
- NLM abbreviation
- NMR Biomed
- ISSN
- 0952-3480
- eISSN
- 1099-1492
- Grant note
- 5 T32 CA009206 / NCI NIH HHS T32 CA009206 / NCI NIH HHS R01DK092454 / NIDDK NIH HHS R01 DK092454 / NIDDK NIH HHS
- Language
- English
- Date published
- 07/2015
- Academic Unit
- Roy J. Carver Department of Biomedical Engineering; Radiology; Electrical and Computer Engineering; Health, Sport, and Human Physiology
- Record Identifier
- 9984274956702771
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