Journal article
Effect of anti-CD4 on CD4 subsets. I: Anti-CD4 preferentially deletes resting, naive CD4 cells and spares activated CD4 cells
The Journal of immunology (1950), Vol.152(2), pp.405-412
1994
DOI: 10.4049/jimmunol.152.2.405
PMID: 7904288
Abstract
Anti-CD4 has been extensively studied in murine models of autoimmunity and transplantation. The timing of anti-CD4 administration in these systems is critical because anti-CD4 effectively blocks primary T-dependent responses but does not diminish ongoing or memory responses in immunized animals. These differential effects suggest that anti-CD4 suppresses a subpopulation of CD4+ cells. We previously observed in vitro that simultaneous activation through TCR-T3 rescued CD4+ cells from anti-CD4 elimination. From this we hypothesized that activated CD4+ cells resisted the effects of anti-CD4. We now show that in vivo treatment with anti-CD4 preferentially eliminated resting, naive CD4+ cells rather than memory and effector CD4+ cells. The CD4+ cells that remained after anti-CD4 treatment exhibited evidence of recent activation, because a higher percentage expressed IL-2R, regardless of subset phenotype. Moreover, Mls-1-primed, anti-CD4-treated mice showed a higher percentage of V beta 6+ (Mls-1 reactive) CD4+ cells than either unprimed mice, anti-CD4-treated mice, or Mls-1-primed controls, implicating the importance of recent activation. These anti-CD4-resistant cells also retained their functional abilities. T cells from BALB/c mice treated with anti-CD4 after Mls-1 immunization maintained their MLR proliferation against DBA/2 stimulator cells. In addition, anti-CD4 did not reduce T-dependent antibody responses in mice previously primed against the Ag cholera toxin or SRBC. Thus, activated CD4+ cells resist the suppressive effects of anti-CD4. Our findings have critical implications for the ongoing clinical trials using anti-CD4.
Details
- Title: Subtitle
- Effect of anti-CD4 on CD4 subsets. I: Anti-CD4 preferentially deletes resting, naive CD4 cells and spares activated CD4 cells
- Creators
- Jacqueline H Chace - Univ. Iowa coll. medicine, dep. medicine, Iowa City IA 52242, United StatesJohn S Cowdery - Univ. Iowa coll. medicine, dep. medicine, Iowa City IA 52242, United StatesElizabeth H Field - Univ. Iowa coll. medicine, dep. medicine, Iowa City IA 52242, United States
- Resource Type
- Journal article
- Publication Details
- The Journal of immunology (1950), Vol.152(2), pp.405-412
- Publisher
- American Association of Immunologists
- DOI
- 10.4049/jimmunol.152.2.405
- PMID
- 7904288
- ISSN
- 0022-1767
- eISSN
- 1550-6606
- Language
- English
- Date published
- 1994
- Academic Unit
- Immunology; Internal Medicine
- Record Identifier
- 9984094484202771
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