Effect of antioxidant phytochemicals on the hepatic tumor promoting activity of 3,3′,4,4′-tetrachlorobiphenyl (PCB-77)

Job C Tharappel; Hans-Joachim Lehmler; Cidambi Srinivasan; Larry W Robertson; Brett T Spear; Howard P Glauert

doi:10.1016/j.fct.2008.08.023

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Effect of antioxidant phytochemicals on the hepatic tumor promoting activity of 3,3′,4,4′-tetrachlorobiphenyl (PCB-77)

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Effect of antioxidant phytochemicals on the hepatic tumor promoting activity of 3,3′,4,4′-tetrachlorobiphenyl (PCB-77)

Job C Tharappel, Hans-Joachim Lehmler, Cidambi Srinivasan, Larry W Robertson, Brett T Spear and Howard P Glauert

Food and chemical toxicology, Vol.46(11), pp.3467-3474

2008

DOI: 10.1016/j.fct.2008.08.023

PMCID: PMC2597727

PMID: 18796325

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http://doi.org/10.1016/j.fct.2008.08.023View

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Abstract

Polychlorinated biphenyls (PCBs) have promoting activity in the liver, which may be brought about in part by the induction of oxidative stress. In this study we examined the effects of several antioxidant phytochemicals on the tumor promoting activity of 3,3′,4′4-tetrachlorobiphenyl (PCB-77). Female Sprague Dawley rats were first injected with diethylnitrosamine (DEN, 150 mg/kg) and one week later the rats were fed an AIN-93 based purified diet or the same diet containing ellagic acid (0.4%), β-carotene (0.5%), curcumin (0.5%), N-acetyl cysteine (NAC, 1.0%), coenzyme CoQ 10 (CoQ 10, 0.4%), resveratrol (0.005%), lycopene (10% as Lycovit, which contains 10% lycopene), or a tea extract (1%, containing 16.5% epigallocatechin-3-gallate [EGCG] and 33.4% total catechins). Rats were fed the diets for the remainder of the study. After three weeks, 2/3 of the control rats and all of the antioxidant diet-fed rats were injected i.p. with PCB-77 (300 μmol/kg) every other week for four injections. All rats were euthanized ten days after the last PCB injection. The rats that received PCB-77 alone showed an increase in the number and size of placental glutathione S-transferase (PGST)-positive foci in the liver. Lycopene significantly decreased the number of foci, while curcumin and CoQ 10 decreased the size of the foci. In contrast, ellagic acid increased the number but decreased the size of the foci. All of the other phytochemicals showed only slight or no effects. Compared with the PCB-77 group, CoQ 10 increased cell proliferation in normal hepatocytes, whereas the other antioxidants had no effect in either normal or PGST-positive hepatocytes. These findings show that none of the antioxidant phytochemicals produced a clear decrease in the promoting activity of PCB-77.

Phytochemicals

Antioxidant

Altered hepatic foci

PCB

Details

Title: Subtitle: Effect of antioxidant phytochemicals on the hepatic tumor promoting activity of 3,3′,4,4′-tetrachlorobiphenyl (PCB-77)
Creators: Job C Tharappel - Graduate Center for Nutritional Sciences, 222 Funkhouser Building, University of Kentucky, Lexington, KY 40506-0054, United States
Hans-Joachim Lehmler - Department of Occupational and Environmental Health, University of Iowa, Iowa City, IA 52242-5000, United States
Cidambi Srinivasan - Department of Statistics, 817 Patterson Office Tower, University of Kentucky, Lexington, KY 40506-0027, United States
Larry W Robertson - Department of Occupational and Environmental Health, University of Iowa, Iowa City, IA 52242-5000, United States
Brett T Spear - Department of Microbiology, Immunology, and Molecular Genetics, University of Kentucky, Lexington, KY 40536-0298, United States
Howard P Glauert - Graduate Center for Nutritional Sciences, 222 Funkhouser Building, University of Kentucky, Lexington, KY 40506-0054, United States
Resource Type: Journal article
Publication Details: Food and chemical toxicology, Vol.46(11), pp.3467-3474
DOI: 10.1016/j.fct.2008.08.023
PMID: 18796325
PMCID: PMC2597727
NLM abbreviation: Food Chem Toxicol
ISSN: 0278-6915
eISSN: 1873-6351
Publisher: Elsevier Ltd
Language: English
Date published: 2008
Academic Unit: Occupational and Environmental Health; Iowa Neuroscience Institute; Iowa Superfund Research Program
Record Identifier: 9984001091202771

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