Journal article
Effect of antioxidant treatment of streptozotocin-induced diabetic rats on endoneurial blood flow, motor nerve conduction velocity, and vascular reactivity of epineurial arterioles of the sciatic nerve
Diabetes (New York, N.Y.), Vol.50(8), pp.1927-1937
2001
DOI: 10.2337/diabetes.50.8.1927
PMID: 11473057
Abstract
We have shown that diabetes-induced reduction in endoneurial blood flow (EBF) and impaired endothelium-dependent vascular relaxation precede slowing of motor nerve conduction velocity (MNCV) and decreased sciatic nerve Na(+)/K(+) ATPase activity. Furthermore, vascular dysfunction was accompanied by an accumulation of superoxide in arterioles that provide circulation to the sciatic nerve. In the present study, we examined the effect that treatment of streptozotocin-induced diabetic rats with antioxidants has on vascular and neural function. Diabetic rats were treated with 0.5% alpha-lipoic acid as a diet supplement or with hydroxyethyl starch deferoxamine (HES-DFO) by weekly intravenous injections at a dose of 75 mg/kg. The treatments significantly improved diabetes-induced decrease in EBF, acetylcholine-mediated vascular relaxation in arterioles that provide circulation to the region of the sciatic nerve, and MNCV. The treatments also reduced the production of superoxide by the aorta and superoxide and peroxynitrite by arterioles that provide circulation to the region of the sciatic nerve. Treating diabetic rats with alpha-lipoic acid prevented the diabetes-induced increase in thiobarbituric acid-reactive substances in serum and significantly improved lens glutathione levels. In contrast, treating diabetic rats with HES-DFO did not prevent diabetes-induced changes of either of these markers of oxidative stress. Diabetes-induced increase in sciatic nerve conjugated diene levels was not improved by treatment with either alpha-lipoic acid or HES-DFO. Treating diabetic rats with alpha-lipoic acid but not HES-DFO partially improved sciatic nerve Na(+)/K(+) ATPase activity and myo-inositol content. The increase in sciatic nerve sorbitol levels in diabetic rats was unchanged by either treatment. These studies suggest that diabetes-induced oxidative stress and the generation of superoxide may be partially responsible for the development of diabetic vascular and neural complications.
Details
- Title: Subtitle
- Effect of antioxidant treatment of streptozotocin-induced diabetic rats on endoneurial blood flow, motor nerve conduction velocity, and vascular reactivity of epineurial arterioles of the sciatic nerve
- Creators
- Lawrence J COPPEY - Diabetes Endocrinology Research Center, Veterans Affairs Medical Center, and the Department of Internal Medicine, University of Iowa, Iowa City, Iowa, United StatesJill S GELLETT - Diabetes Endocrinology Research Center, Veterans Affairs Medical Center, and the Department of Internal Medicine, University of Iowa, Iowa City, Iowa, United StatesEric P DAVIDSON - Diabetes Endocrinology Research Center, Veterans Affairs Medical Center, and the Department of Internal Medicine, University of Iowa, Iowa City, Iowa, United StatesJoyce A DUNLAP - Diabetes Endocrinology Research Center, Veterans Affairs Medical Center, and the Department of Internal Medicine, University of Iowa, Iowa City, Iowa, United StatesDonald D LUND - Diabetes Endocrinology Research Center, Veterans Affairs Medical Center, and the Department of Internal Medicine, University of Iowa, Iowa City, Iowa, United StatesMark A YOREK - Diabetes Endocrinology Research Center, Veterans Affairs Medical Center, and the Department of Internal Medicine, University of Iowa, Iowa City, Iowa, United States
- Resource Type
- Journal article
- Publication Details
- Diabetes (New York, N.Y.), Vol.50(8), pp.1927-1937
- Publisher
- American Diabetes Association
- DOI
- 10.2337/diabetes.50.8.1927
- PMID
- 11473057
- ISSN
- 0012-1797
- eISSN
- 1939-327X
- Language
- English
- Date published
- 2001
- Academic Unit
- Fraternal Order of Eagles Diabetes Research Center; Endocrinology and Metabolism; Internal Medicine
- Record Identifier
- 9984094720302771
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