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Effect of apical chloride concentration on the measurement of responses to CFTR modulation in airway epithelia cultured from nasal brushings
Journal article   Open access   Peer reviewed

Effect of apical chloride concentration on the measurement of responses to CFTR modulation in airway epithelia cultured from nasal brushings

Preston E. Bratcher, Sangya Yadav, Ciaran A. Shaughnessy, Ian M. Thornell and Pamela L. Zeitlin
Physiological reports, Vol.8(19), pp.e14603-n/a
10/10/2020
DOI: 10.14814/phy2.14603
PMCID: PMC7547589
PMID: 33038073
url
https://doi.org/10.14814/phy2.14603View
Published (Version of record) Open Access

Abstract

CFTR‐mediated chloride conductance in airway epithelia is commonly analyzed in the presence of a chloride gradient, which increases CFTR‐mediated changes in potential difference and transepithelial current. This study demonstrates that the presence of a chloride gradient can impact measurements of responses of CF epithelial to CFTR modulation in vitro.
CFTR modulator chloride gradient Cystic fibrosis in vitro efficacy Original Research

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