Journal article
Effect of benzalkonium chloride on dentin bond strength and endogenous enzymatic activity
Journal of dentistry, Vol.85, pp.25-32
06/01/2019
DOI: 10.1016/j.jdent.2019.04.008
PMID: 30998949
Abstract
Objective: This in vitro study evaluated at baseline (TO) and over time (T12 months), the effect of a mull-mode universal adhesive compared with two experimental formulations blended with different concentrations of benzalkonium chloride (BAC), on bond strength and endogenous enzymatic activity.
Methods and materials: Specimens were assigned to the following groups according to the adhesive protocol: Gl) All-Bond Universal (ABU) self-etch (SE); G2) ABU + 0.5% BAC SE; G3) ABU + 1% methacrylate BAC SE; G4) ABU etch-and-rinse (E & R); G5) ABU + 0.5% BAC E & R; G6) ABU + 1% methacrylate BAC HER. Gelatin zymography was performed on dentin powder obtained from eight human third molars. Endogenous enzymatic activity within the hybrid layer was examined using in situ zymography after 24h (T0) or 1-year storage in artificial saliva (T12). Forty intact molars were prepared for microtensile bond strength test at TO and T12. Results were statistically analyzed with three-way ANOVA (alpha = 0.05).
Results: Gelatin zymography assay and in situ zymography quantification analyses indicated that all the BAC-containing formulations decreased matrix metalloproteinase expression. However, in situ zymography showed a general trend of enzymatic activity increase after aging. Microtensile bond-strength testing showed decrease in bond strength over time in all the tested groups; performances of the 1% methacrylate BAC experimental groups were worse than the control.
Conclusions: BAC-containing adhesives reduce endogenous enzymatic activity both immediately and over time. However, independently from the adhesive employed, increase in the gelatinolytic activity over time and decrease in bond strength was found (especially in the BAC + 1% methacrylate groups), probably due to impaired polymerization properties.
Clinical significance: Adhesives containing protease inhibitors are practical and efficient tools in clinical practice for enhancement of the longevity of dental restorations. However, extensive investigation of the mechanical and adhesive properties of the material is necessary prior to their clinical use.
Details
- Title: Subtitle
- Effect of benzalkonium chloride on dentin bond strength and endogenous enzymatic activity
- Creators
- Allegra Comba - University of BolognaTatjana Maravic - University of BolognaLucrezia Valente - University of BolognaMargherita Girlando - University of BolognaSandra R. Cunha - Universidade de São PauloVittorio Checchi - University of BolognaStefano Salgarello - University of BresciaFranklin R. Tay - Augusta UniversityNicola Scotti - University of TurinLorenzo Breschi - University of BolognaAnnalisa Mazzoni - University of Bologna
- Resource Type
- Journal article
- Publication Details
- Journal of dentistry, Vol.85, pp.25-32
- DOI
- 10.1016/j.jdent.2019.04.008
- PMID
- 30998949
- NLM abbreviation
- J Dent
- ISSN
- 0300-5712
- eISSN
- 1879-176X
- Publisher
- Elsevier
- Number of pages
- 8
- Language
- English
- Date published
- 06/01/2019
- Academic Unit
- Operative Dentistry
- Record Identifier
- 9984696753902771
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