Journal article
Effect of combination therapy consisting of enalapril, α-lipoic acid, and menhaden oil on diabetic neuropathy in a high fat/low dose streptozotocin treated rat
European journal of pharmacology, Vol.765, pp.258-267
10/15/2015
DOI: 10.1016/j.ejphar.2015.08.015
PMID: 26291662
Abstract
We have previously demonstrated that treating diabetic rats with enalapril, an angiotensin converting enzyme (ACE) inhibitor, α-lipoic acid, an antioxidant, or menhaden oil, a natural source of omega-3 fatty acids can partially improve diabetic peripheral neuropathy. In this study we sought to determine the efficacy of combining these three treatments on vascular and neural complications in a high fat fed low dose streptozotocin treated rat, a model of type 2 diabetes. Rats were fed a high fat diet for 8 weeks followed by a 30mg/kg dose of streptozotocin. Eight weeks after the onset of hyperglycemia diabetic rats were treated with a combination of enalapril, α-lipoic acid and menhaden oil. Diabetic rats not receiving treatment were continued on the high fat diet. Glucose clearance was impaired in diabetic rats and significantly improved with treatment. Diabetes caused steatosis, elevated serum lipid levels, slowing of motor and sensory nerve conduction, thermal hypoalgesia, reduction in intraepidermal nerve fiber profiles, decrease in cornea sub-basal nerve fiber length and corneal sensitivity and impairment in vascular relaxation to acetylcholine and calcitonin gene-related peptide in epineurial arterioles of the sciatic nerve. Treating diabetic rats with the combination of enalapril, α-lipoic acid and menhaden oil reversed all these deficits to near control levels except for motor nerve conduction velocity which was also significantly improved compared to diabetic rats but remained significantly decreased compared to control rats. These studies suggest that a combination therapeutic approach may be most effective for treating vascular and neural complications of type 2 diabetes.
Details
- Title: Subtitle
- Effect of combination therapy consisting of enalapril, α-lipoic acid, and menhaden oil on diabetic neuropathy in a high fat/low dose streptozotocin treated rat
- Creators
- Eric P. Davidson - University of IowaAmey Holmes - Iowa City VA Health Care SystemLawrence J. Coppey - University of IowaMark A. Yorek - University of Iowa
- Resource Type
- Journal article
- Publication Details
- European journal of pharmacology, Vol.765, pp.258-267
- Publisher
- Elsevier B.V
- DOI
- 10.1016/j.ejphar.2015.08.015
- PMID
- 26291662
- ISSN
- 0014-2999
- eISSN
- 1879-0712
- Grant note
- name: Department of Veterans Affairs, Veterans Health Administration, Office of Research and Development, Biomedical Laboratory Research and Development, award: BX001680–01; DOI: 10.13039/100006380, name: Rehabilitation Research and Development Service, award: RX000889–01; name: Iowa City VA Center of Excellence for the Prevention and Treatment of Visual Loss, award: C9251-C
- Language
- English
- Date published
- 10/15/2015
- Academic Unit
- Fraternal Order of Eagles Diabetes Research Center; Endocrinology and Metabolism; Internal Medicine
- Record Identifier
- 9984359893602771
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