Effect of diet-induced obesity or type 1 or type 2 diabetes on corneal nerves and peripheral neuropathy in C57Bl/6J mice

Matthew S Yorek; Alexander Obrosov; Hanna Shevalye; Amey Holmes; Matthew M Harper; Randy H Kardon; Mark A Yorek

doi:10.1111/jns.12111

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Effect of diet-induced obesity or type 1 or type 2 diabetes on corneal nerves and peripheral neuropathy in C57Bl/6J mice

Journal article

Open access

Peer reviewed

Effect of diet-induced obesity or type 1 or type 2 diabetes on corneal nerves and peripheral neuropathy in C57Bl/6J mice

Matthew S Yorek, Alexander Obrosov, Hanna Shevalye, Amey Holmes, Matthew M Harper, Randy H Kardon and Mark A Yorek

Journal of the peripheral nervous system, Vol.20(1), pp.24-31

03/2015

DOI: 10.1111/jns.12111

PMCID: PMC4470853

PMID: 25858759

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Abstract

We determined the impact diet-induced obesity (DIO) and types 1 and 2 diabetes have on peripheral neuropathy with emphasis on corneal nerve structural changes in C57Bl/6J mice. Endpoints examined included nerve conduction velocity, response to thermal and mechanical stimuli and innervation of the skin and cornea. DIO mice and to a greater extent type 2 diabetic mice were insulin resistant. DIO and both types 1 and 2 diabetic mice developed motor and sensory nerve conduction deficits. In the cornea of DIO and type 2 diabetic mice there was a decrease in sub-epithelial corneal nerves, innervation of the corneal epithelium, and corneal sensitivity. Type 1 diabetic mice did not present with any significant changes in corneal nerve structure until after 20 weeks of hyperglycemia. DIO and type 2 diabetic mice developed corneal structural damage more rapidly than type 1 diabetic mice although hemoglobin A1 C values were significantly higher in type 1 diabetic mice. This suggests that DIO with or without hyperglycemia contributes to development and progression of peripheral neuropathy and nerve structural damage in the cornea.

Glucose Tolerance Test

Diet - adverse effects

Mice, Inbred C57BL

Neural Conduction - physiology

Aldehydes - metabolism

Tyrosine - analogs & derivatives

Tyrosine - metabolism

Animals

Diabetes Mellitus, Experimental - etiology

Diabetic Neuropathies - pathology

Cornea - innervation

Obesity - etiology

Cornea - pathology

Mice

Diabetic Neuropathies - physiopathology

Ganglia, Spinal - metabolism

Details

Title: Subtitle: Effect of diet-induced obesity or type 1 or type 2 diabetes on corneal nerves and peripheral neuropathy in C57Bl/6J mice
Creators: Matthew S Yorek - Veterans Affairs Center for the Prevention and Treatment of Visual Loss, Iowa City, IA, USA
Alexander Obrosov - Department of Internal Medicine, University of Iowa, Iowa City, IA, USA
Hanna Shevalye - Department of Internal Medicine, University of Iowa, Iowa City, IA, USA
Amey Holmes - Department of Veterans Affairs Iowa City Health Care System, Iowa City, IA, USA
Matthew M Harper - Department of Ophthalmology and Visual Sciences, University of Iowa, Iowa City, IA, USA
Randy H Kardon - Department of Ophthalmology and Visual Sciences, University of Iowa, Iowa City, IA, USA
Mark A Yorek - Fraternal Order of Eagles Diabetes Research Center, University of Iowa, Iowa City, IA, USA
Resource Type: Journal article
Publication Details: Journal of the peripheral nervous system, Vol.20(1), pp.24-31
DOI: 10.1111/jns.12111
PMID: 25858759
PMCID: PMC4470853
NLM abbreviation: J Peripher Nerv Syst
ISSN: 1529-8027
eISSN: 1529-8027
Publisher: United States
Grant note: I01 BX001680 / BLRD VA DK081147 / NIDDK NIH HHS R01 DK081147 / NIDDK NIH HHS I01 RX000889 / RRD VA
Language: English
Date published: 03/2015
Academic Unit: Iowa Neuroscience Institute; Biology; Internal Medicine; Ophthalmology and Visual Sciences
Record Identifier: 9983980064702771

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