Journal article
Effect of doxorubicin, daunorubicin, and idarubicin on the growth rate of human leukemia cells (K562) studied with image analysis
Leukemia, Vol.7(6), pp.872-877
1993
PMID: 8501981
Abstract
The effects of doxorubicin, daunorubicin, and idarubicin on the growth of K562 cells were investigated by monitoring the formation of individual colonies in semi-solid culture employing an automated image analysis system. Drug effects were evaluated using short-term exposure (2 h) at clinically achievable drug plasma concentrations. Following drug exposure, heterogeneity in growth patterns was observed which was categorized into three distinct types; (1) continuous growth at a decreased growth rate; (2) limited growth; (3) no growth. In addition to a concentration-dependent decrease of the growth fraction, a reduction of the growth rate of the continuously growing colonies was observed. At equitoxic drug concentrations, comparable changes in growth rate were observed for each of the three anthracyclines studied. It is demonstrated that the fraction of cells which escape drug-induced growth arrest with therapeutically achievable drug concentrations display a significant decrease in growth rate.
Details
- Title: Subtitle
- Effect of doxorubicin, daunorubicin, and idarubicin on the growth rate of human leukemia cells (K562) studied with image analysis
- Creators
- H Minderman - Roswell Park Cancer InstituteH. K Slocum - Roswell Park Cancer InstituteM Malmberg - Roswell Park Cancer InstituteC Haanen - Roswell Park Cancer InstituteY. M Rustum - Roswell Park Cancer Institute
- Resource Type
- Journal article
- Publication Details
- Leukemia, Vol.7(6), pp.872-877
- Publisher
- Nature Publishing
- PMID
- 8501981
- ISSN
- 0887-6924
- eISSN
- 1476-5551
- Language
- English
- Date published
- 1993
- Academic Unit
- Hematology, Oncology, and Blood & Marrow Transplantation; Internal Medicine
- Record Identifier
- 9984360039402771
Metrics
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