Journal article
Effect of enamel microabrasion on bacterial colonization
American journal of dentistry, Vol.10(6), pp.272-274
12/01/1997
PMID: 9590914
Abstract
PURPOSETo determine if microabraded surfaces inhibit Streptococcus mutans colonization.MATERIALS AND METHODSTen extracted molars were obtained and sectioned in half mesio-distally. The mesial side was treated with microabrasion (PREMA Compound). The distal surface was designated as the control site. A plastic tube was secured on each surface with resin composite, exposing a surface of 13.2 mm 2. S. mutans 10,499 was cultured in TSB-YE supplemented with 5% sucrose and 2.5% glucose was added to each secured site in addition to 50 microliters of cell suspension. Samples were incubated in a 5% CO2 chamber for 7 days at 37 degrees C. Samples were taken from each site, incubated with dextranase (75 micrograms 1 ml), sonicated, and vortexed to disrupt cellular aggregates as much as possible. Samples were then plated on Mitis-Salivarius agar using a spiral plating system. Enumeration of numbers of colonizing bacteria was achieved following standard spiral plating system methodology.RESULTSThe mean for the microabraded group was 1.12 x 10(2) cfu/tooth and for the control it was 1.15 x 10(4). Analyzing the data revealed a significant difference (P = 0.0188).
Details
- Title: Subtitle
- Effect of enamel microabrasion on bacterial colonization
- Creators
- A SeguraK J DonlyJ S WefelD Drake
- Resource Type
- Journal article
- Publication Details
- American journal of dentistry, Vol.10(6), pp.272-274
- PMID
- 9590914
- ISSN
- 0894-8275
- Language
- English
- Date published
- 12/01/1997
- Academic Unit
- Endodontics; Dental Research
- Record Identifier
- 9984367632502771
Metrics
3 Record Views