Journal article
Effect of ethanol on neurotrophin-mediated cell survival and receptor expression in cultures of cortical neurons
Brain research. Developmental brain research, Vol.108(1), pp.139-145
1998
DOI: 10.1016/S0165-3806(98)00043-1
PMID: 9693792
Abstract
The interaction of ethanol and neurotrophin-mediated cell survival was examined in primary cultures of cortical neurons. Cells were obtained from rat fetuses on gestational day 16 and maintained in a medium supplemented with either 10% or 1.0% fetal calf serum (FCS). Exogenous nerve growth factor (NGF; 20 ng/ml), brain-derived neurotrophic factor (BDNF; 20 ng/ml) or neurotrophin 3 (NT-3; 20 ng/ml) was added to the cultures alone, or in combination with ethanol (400 mg/dl). The number of viable neurons was determined after a 48 h treatment with a growth factor and/or ethanol. The effects of ethanol on the expression of high affinity neurotrophin receptors (
trkA,
trkB, and
trkC) and the low-affinity receptor (p75), were analyzed using Western immunoblots. In untreated cultures, 22.7% and 26.3% of the cells raised in a medium containing 10% and 1.0% FCS, respectively, were lost. Only NGF prevented the death of the cultured cortical neurons. Ethanol was toxic; it caused a 23.5% and 16.7% loss of cells (for cells grown in a medium containing 10% and 1.0% FCS, respectively) beyond that occurring `naturally' in an untreated culture. Ethanol completely blocked the NGF-mediated cell survival. In general, BDNF and NT-3 did not offset the toxic effect of ethanol. Immunoblotting studies showed that the expression of p75 was significantly (
p<0.05) lower (40%) in ethanol-treated cultures, but ethanol did not affect
trk expression. Thus, ethanol has specific effects upon NGF-mediated cell survival and the effects on the low affinity receptor imply that p75 specifically plays an important role in NGF signaling.
Details
- Title: Subtitle
- Effect of ethanol on neurotrophin-mediated cell survival and receptor expression in cultures of cortical neurons
- Creators
- Gail K Seabold - University of IowaJia Luo - University of IowaMichael W Miller - University of Iowa
- Resource Type
- Journal article
- Publication Details
- Brain research. Developmental brain research, Vol.108(1), pp.139-145
- Publisher
- Elsevier B.V
- DOI
- 10.1016/S0165-3806(98)00043-1
- PMID
- 9693792
- ISSN
- 0165-3806
- Language
- English
- Date published
- 1998
- Academic Unit
- Pathology
- Record Identifier
- 9984186577502771
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