Journal article
Effect of expression of manganese superoxide dismutase in baculovirus-infected insect cells
Applied biochemistry and biotechnology, Vol.119(2), pp.181-193
11/2004
DOI: 10.1385/ABAB:119:2:181
PMID: 15531788
Abstract
It has previously been demonstrated that baculovirus infection of the Spodoptera frugiperda Sf-9 (Sf-9) and Trichoplusia ni BTI-Tn-5B1-4 (Tn-5B1-4) insect cell lines leads to oxidative stress as measured by protein and membrane lipid oxidation and that this oxidative damage contributes to cell death. As a result of these findings, it was hypothesized that baculovirus infection stimulates superoxide radical (O
2
·—
synthesis in the mitochondria and that the resulting O
2
·—
accumulation overwhelms the cells’ antioxidant defenses. We investigated the ability of manganese superoxide dismutase (MnSOD) expression (which reduces O
2
·—
to H2O2) to overcome the oxidative damage caused by baculovirus infection. It was found that MnSOD expression significantly reduced oxidative damage in baculovirus-infected Tn-5B1-4 cells but had no significant effect on oxidative damage in baculovirus-infected Sf-9 cells. The results are consistent with the hypothesis that O
2
·—
accumulation in the mitochondria is at least partially responsible for the oxidative damage resulting from the baculovirus infection of insect cells.
Details
- Title: Subtitle
- Effect of expression of manganese superoxide dismutase in baculovirus-infected insect cells
- Creators
- Ying Wang - University of IowaLarry Oberley - University of IowaDale Howe - University of WyomingDonald Jarvis - University of WyomingGaurav Chauhan - University of IowaDavid Murhammer - University of Iowa
- Resource Type
- Journal article
- Publication Details
- Applied biochemistry and biotechnology, Vol.119(2), pp.181-193
- Publisher
- Humana Press
- DOI
- 10.1385/ABAB:119:2:181
- PMID
- 15531788
- ISSN
- 0273-2289
- eISSN
- 1559-0291
- Language
- English
- Date published
- 11/2004
- Academic Unit
- Chemical and Biochemical Engineering
- Record Identifier
- 9984197081002771
Metrics
5 Record Views