Journal article
Effect of fatty acid composition on insulin and IGF-I binding in retinoblastoma cells
Investigative ophthalmology & visual science, Vol.30(10), pp.2087-2092
1989
PMID: 2676896
Abstract
Receptors for insulin and other hormones are often influenced by their environment, including fatty acid content and membrane fluidity. Y79 retinoblastoma cells enriched with arachidonic or docosahexaenoic acid show an increase in membrane fluidity determined by fluorescence polarization using the plasma membrane-specific probe, trimethylammonium-diphenylhexatriene. Unlike cells enriched with polyunsaturated fatty acids, cells cultured in media containing palmitic or oleic acid showed no changes in membrane fluidity or fatty acid composition. Cells enriched with docosahexaenoic acid show an increase in insulin binding at 15 degrees C that is due to an increase in the number of available insulin receptor sites on the plasma membrane. In contrast, insulin binding is not altered in cells enriched with arachidonic, palmitic or oleic acid. Furthermore, the binding of insulin-like growth factor-I (IGF-I) in Y79 cells is not changed by the fatty acid unsaturation of the cell membrane. Because docosahexaenoic acid is naturally present in high concentrations in the retina these studies suggest it may have a role in modulating insulin binding and function.
Details
- Title: Subtitle
- Effect of fatty acid composition on insulin and IGF-I binding in retinoblastoma cells
- Creators
- Mark Yorek - Veterans administration medical cent., Iowa City IA 52246, United StatesEllen Leeney - Veterans administration medical cent., Iowa City IA 52246, United StatesJoyce Dunlap - Veterans administration medical cent., Iowa City IA 52246, United StatesBarry Ginsberg - Veterans administration medical cent., Iowa City IA 52246, United States
- Resource Type
- Journal article
- Publication Details
- Investigative ophthalmology & visual science, Vol.30(10), pp.2087-2092
- Publisher
- Association for Research in Vision and Ophtalmology
- PMID
- 2676896
- ISSN
- 0146-0404
- eISSN
- 1552-5783
- Language
- English
- Date published
- 1989
- Academic Unit
- Fraternal Order of Eagles Diabetes Research Center; Endocrinology and Metabolism; Internal Medicine
- Record Identifier
- 9984094670802771
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