Journal article
Effect of glucose on uptake of radiolabeled glucose, 2-DG, and 3-O-MG by the perfused rat liver
The American journal of physiology, Vol.271(2 Pt 1), pp.E384-396
08/1996
DOI: 10.1152/ajpendo.1996.271.2.E384
PMID: 8770034
Abstract
In the transition from the fasting to the fed state, plasma glucose levels rise, and the liver converts from an organ producing glucose to one of storage. To determine the effect of glucose on hepatic glucose uptake, radiolabeled glucose, 2-deoxyglucose, and 3-O-methylglucose were injected into perfused rat livers during different nontracer glucose levels, and the concentrations in the outflow were measured. A mathematical model was developed that described the behavior of the injected compounds as they traveled through the liver and was used to simulate and fit the experimental results. The rates of membrane transport, glucokinase, glucose-6-phosphatase, and the consumption of glucose 6-phosphate were estimated. Membrane transport for all of the tracers decreased as nontracer glucose increased, demonstrating competitive inhibition of the glucose transporter. In contrast, the consumption of injected [2-14C]glucose increased when glucose was elevated, demonstrating that glucose caused an activation of enzyme activity that overcame the competitive inhibition of transport and phosphorylation. When glucose was elevated, the rate coefficient of glucokinase did not decrease, indicating that glucokinase was stimulated by glucose. Both changes would lead to the increased glycogen synthesis and decreased glucose production rate observed in vivo during the fasted-to-fed transition.
Details
- Title: Subtitle
- Effect of glucose on uptake of radiolabeled glucose, 2-DG, and 3-O-MG by the perfused rat liver
- Creators
- Ian R Sweet - Center for Bioengineering, University of Washington, Seattle 98195, USALanell M PetersonKeith KrollC J GoodnerMarianne BerryMichael M Graham
- Resource Type
- Journal article
- Publication Details
- The American journal of physiology, Vol.271(2 Pt 1), pp.E384-396
- Publisher
- United States
- DOI
- 10.1152/ajpendo.1996.271.2.E384
- PMID
- 8770034
- ISSN
- 0002-9513
- eISSN
- 2163-5773
- Language
- English
- Date published
- 08/1996
- Academic Unit
- Radiology; Radiation Oncology
- Record Identifier
- 9984047772402771
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