Effect of glycemic control on corneal nerves and peripheral neuropathy in streptozotocin-induced diabetic C57Bl/6J mice

Matthew S Yorek; Alexander Obrosov; Hanna Shevalye; Sergey Lupachyk; Matthew M Harper; Randy H Kardon; Mark A Yorek

doi:10.1111/jns.12086

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Effect of glycemic control on corneal nerves and peripheral neuropathy in streptozotocin-induced diabetic C57Bl/6J mice

Journal article

Peer reviewed

Effect of glycemic control on corneal nerves and peripheral neuropathy in streptozotocin-induced diabetic C57Bl/6J mice

Matthew S Yorek, Alexander Obrosov, Hanna Shevalye, Sergey Lupachyk, Matthew M Harper, Randy H Kardon and Mark A Yorek

Journal of the peripheral nervous system, Vol.19(3), pp.205-217

09/2014

DOI: 10.1111/jns.12086

PMCID: PMC4986703

PMID: 25403729

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Abstract

We sought to determine the impact that duration of hyperglycemia and control has on corneal nerve fiber density in relation to standard diabetic neuropathy endpoints. Control and streptozotocin-diabetic C57Bl/6J mice were analyzed after 4, 8, 12, and 20 weeks. For the 20-week time point, five groups of mice were compared: control, untreated diabetic, and diabetic treated with insulin designated as having either poor glycemic control, good glycemic control, or poor glycemic control switched to good glycemic control. Hyperglycemia was regulated by use of insulin-releasing pellets. Loss of corneal nerves in the sub-epithelial nerve plexus or corneal epithelium progressed slowly in diabetic mice requiring 20 weeks to reach statistical significance. In comparison, slowing of motor and sensory nerve conduction velocity developed rapidly with significant difference compared with control mice observed after 4 and 8 weeks of hyperglycemia, respectively. In diabetic mice with good glycemic control, average blood glucose levels over the 20-week experimental period were lowered from 589 ± 2 to 251 ± 9 mg/dl. All diabetic neuropathy endpoints examined were improved in diabetic mice with good glycemic control compared with untreated diabetic mice. However, good control of blood glucose was not totally sufficient in preventing diabetic neuropathy.

Insulin - pharmacology

Diabetes Mellitus, Experimental - drug therapy

Diabetic Neuropathies - etiology

Mice, Inbred C57BL

Hyperglycemia - complications

Hyperglycemia - drug therapy

Hypoglycemic Agents - pharmacology

Animals

Hyperglycemia - chemically induced

Nerve Fibers - physiology

Cornea - innervation

Diabetes Mellitus, Experimental - chemically induced

Diabetes Mellitus, Experimental - complications

Epithelium, Corneal - innervation

Mice

Streptozocin - toxicity

Anti-Bacterial Agents - toxicity

Diabetic Neuropathies - physiopathology

Details

Title: Subtitle: Effect of glycemic control on corneal nerves and peripheral neuropathy in streptozotocin-induced diabetic C57Bl/6J mice
Creators: Matthew S Yorek - Department of Veterans Affairs, Iowa City Health Care System, Iowa City, IA, USA; Veterans Affairs Center for the Prevention and Treatment of Visual Loss, Iowa City, IA, USA
Alexander Obrosov
Hanna Shevalye
Sergey Lupachyk
Matthew M Harper
Randy H Kardon
Mark A Yorek
Resource Type: Journal article
Publication Details: Journal of the peripheral nervous system, Vol.19(3), pp.205-217
DOI: 10.1111/jns.12086
PMID: 25403729
PMCID: PMC4986703
NLM abbreviation: J Peripher Nerv Syst
ISSN: 1529-8027
eISSN: 1529-8027
Publisher: United States
Grant note: I01 BX001680 / BLRD VA DK081147 / NIDDK NIH HHS R01 DK081147 / NIDDK NIH HHS I01 RX000889 / RRD VA
Language: English
Date published: 09/2014
Academic Unit: Iowa Neuroscience Institute; Biology; Internal Medicine; Ophthalmology and Visual Sciences
Record Identifier: 9983980066602771

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