Effect of hypocapnia/alkalosis on the fluid filtration rate in isolated and perfused rabbit lungs

Daniela Urich; Humberto Trejo; Alejandro Pezzulo; Juan Carlos Caraballo; Jeydith Gutiérrez; Ignacio Castro; Roberto Sánchez-de León

Effect of hypocapnia/alkalosis on the fluid filtration rate in isolated and perfused rabbit lungs

Journal article

Peer reviewed

Effect of hypocapnia/alkalosis on the fluid filtration rate in isolated and perfused rabbit lungs

Daniela Urich, Humberto Trejo, Alejandro Pezzulo, Juan Carlos Caraballo, Jeydith Gutiérrez, Ignacio Castro and Roberto Sánchez-de León

Investigación clínica, Vol.49(2), pp.181-193

06/2008

PMID: 18717265

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Abstract

Hypocapnia/alkalosis is a consequence of several lung and metabolic pathologies. The aim of this study was to determine whether the increase of fluid filtration rate (FFR) that occurs during Hypocapnia/alkalosis circumstances is determined by hypocapnia, alkalosis or both. 7 groups were formed (N=36) using isolated rabbit lungs. Group 1: Control (PCO2 6%, pH: 7.35-7.45); Group 2 (n=6): Hypocapnia/Alkalosis (CO2 1%, pH: 7.9); Group 3 (n=6): Hypocapnia/Normo-pH (CO2 1% pH 7.35-7.45), Group 4 (n=6) Normocapnia/Alcalosis (CO2 6%, pH: 7.9). Fenoterol, papaverine and hydrocortisone were added to Groups 5, 6 and 7 (n=4) respectively, all under Normocapnia/Alkalosis. FFR and Pulmonary Arterial Pressure (Pap) were considerably higher in group 2 than in control (FFR: 1.92g/min +/- 0.6 vs 0.0 g/min +/- 0.006). A strong influence exerted by pH was observed when Group 3 and group 4 were compared (FFR: 0.02 g/min +/- 0.009 vs 2.3 g/min +/- 0.9) and (Pap: 13.5 cmH2O +/- 1.4 vs 90 cmH2O +/- 15). A reduced effect was observed in groups 5 and 6 (papaverine and hydrocorisone) and a totally abolished effect was observed in group 7 (fenoterol) (FFR: 0.001 +/- 0.0003 mL/min and Pap: 14 +/- 0.8 cmH2O). Pulmonary edema induced by Hypocapnia/alkalosis is a consequence of alkalosis and not of hypocapnia. This effect could be due to inflammatory damage in the lung parenchyma and alkalosis-mediated vasoconstriction.

Pulmonary Edema - etiology

Pulmonary Edema - physiopathology

Vasoconstriction - physiology

Fluid Shifts - physiology

Alkalosis - complications

Hypocapnia - complications

Blood Pressure - drug effects

Rabbits

Hypocapnia - physiopathology

Fenoterol - pharmacology

Vasodilator Agents - pharmacology

Anti-Inflammatory Agents - pharmacology

Adrenergic beta-Agonists - pharmacology

Papaverine - pharmacology

Lung - physiopathology

Pulmonary Artery

Vasoconstriction - drug effects

Alkalosis - physiopathology

Animals

Perfusion

Lung - drug effects

Hydrocortisone - pharmacology

Fluid Shifts - drug effects

Lung - blood supply

Hydrogen-Ion Concentration

Details

Title: Subtitle: Effect of hypocapnia/alkalosis on the fluid filtration rate in isolated and perfused rabbit lungs
Creators: Daniela Urich - Sección de Fisiología Respiratoria, Facultad de Medicina, Escuela de Medicina Luis Razetti, Universidad Central de Venezuela, Caracas, Venezuela
Humberto Trejo
Alejandro Pezzulo
Juan Carlos Caraballo
Jeydith Gutiérrez
Ignacio Castro
Roberto Sánchez-de León
Resource Type: Journal article
Publication Details: Investigación clínica, Vol.49(2), pp.181-193
PMID: 18717265
NLM abbreviation: Invest Clin
ISSN: 0535-5133
Alternative title: Efecto de la hipocapnia/alcalosis sobre la tasa de filtración de liquidos en pulmones aislados y perfundidos de conejos
Language: Spanish
Date published: 06/2008
Academic Unit: Pulmonary, Critical Care, and Occupational Medicine; Iowa Neuroscience Institute; General Internal Medicine; Internal Medicine
Record Identifier: 9984094637302771

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