Journal article
Effect of increased concentration of D-glucose or L-fucose on monocyte adhesion to endothelial cell monolayers and activation of nuclear factor-kappaB
Metabolism, clinical and experimental, Vol.51(2), pp.225-234
02/2002
DOI: 10.1053/meta.2002.29958
PMID: 11833053
Abstract
Increased adhesion of monocytes to endothelial cells has been linked to the development and progression of atherosclerosis in humans with diabetes. Previous studies have shown that increased concentration of glucose and subsequent generation of reactive oxygen species and the activation of the transcription factor nuclear factor-kappaB (NF-kappaB) may mediate this response. However, our studies have shown that in addition to glucose, other monosaccharides, such as L-fucose, which is increased in circulation in diabetes, may also contribute to the development of diabetic complications. In these studies, we examined the effect of an increased concentration of L-fucose on monocyte adhesion to cultured bovine aorta endothelial cells. Exposing cultured bovine aorta endothelial cells to an increased concentration of either glucose or L-fucose caused a concentration-dependent increase in adhesion of monocytes. The increase in monocyte adhesion induced by glucose or L-fucose was preceded by the activation to NF-kappaB and the generation of reactive oxygen species. The combination of glucose and L-fucose at a submaximal concentration did not appear to have an additive effect on the induction of monocyte adhesion. The addition of alpha-lipoic acid partially prevented the glucose and L-fucose-induced activation of NF-kappaB, generation of reactive oxygen species, and increase in monocyte adhesion. This suggests that the effect of an increased concentration of glucose or L-fucose on monocyte adhesion to endothelial cells is at least partially due to the production oxygen-derived free radicals. Furthermore, these studies provide evidence that monosaccharides other than glucose that are increased in the circulation of humans with diabetes may contribute to vascular defects in diabetes.
Details
- Title: Subtitle
- Effect of increased concentration of D-glucose or L-fucose on monocyte adhesion to endothelial cell monolayers and activation of nuclear factor-kappaB
- Creators
- Mark A Yorek - Department of Internal Medicine, Diabetes-Endocrinology Research Center and Veterans Affairs Medical Center, University of Iowa, Iowa City, IA 52246, USAJoyce A Dunlap
- Resource Type
- Journal article
- Publication Details
- Metabolism, clinical and experimental, Vol.51(2), pp.225-234
- DOI
- 10.1053/meta.2002.29958
- PMID
- 11833053
- ISSN
- 0026-0495
- eISSN
- 1532-8600
- Grant note
- DK25295 / NIDDK NIH HHS
- Language
- English
- Date published
- 02/2002
- Academic Unit
- Fraternal Order of Eagles Diabetes Research Center; Endocrinology and Metabolism; Internal Medicine
- Record Identifier
- 9984094758302771
Metrics
17 Record Views