Journal article
Effect of mitoquinone on liver metabolism and steatosis in obese and diabetic rats
Pharmacology research & perspectives, Vol.9(1), pp.e00701-n/a
02/06/2021
DOI: 10.1002/prp2.701
PMCID: PMC7866483
PMID: 33547885
Abstract
Previous work by ourselves and others showed that mitoquinone (mitoQ) reduced oxidative damage and prevented hepatic fat accumulation in mice made obese with high‐fat (HF) feeding. Here we extended these studies to examine the effect of mitoQ on parameters affecting liver function in rats treated with HF to induce obesity and in rats treated with HF plus streptozotocin (STZ) to model a severe form of type 2 diabetes. In prior reported work, we found that mitoQ significantly improved glycemia based on glucose tolerance data in HF rats but not in the diabetic rats. Here we found only non‐significant reductions in insulin and glucose measured in the fed state at sacrifice in the HF mice treated with mitoQ. Metabolomic data showed that mitoQ altered several hepatic metabolic pathways in HF‐fed obese rats toward those observed in control normal chow‐fed non‐obese rats. However, mitoQ had little effect on pathways observed in the diabetic rats, wherein diabetes itself induced marked pathway aberrations. MitoQ did not alter respiration or membrane potential in isolated liver mitochondria. MitoQ reduced liver fat and liver hydroperoxide levels but did not improve liver function as marked by circulating levels of aspartate and alanine aminotransferase (ALT). In summary, our results for HF‐fed rats are consistent with past findings in HF‐fed mice indicating decreased liver lipid hydroperoxides (LPO) and improved glycemia. However, in contrast to the HF obese mice, mitoQ did not improve glycemia or reset perturbed metabolic pathways in the diabetic rats.
High fat fed obese and high fat fed streptozotocin (STZ) diabetic rats were treated with mitoquinone (MQ) or vehicle.
Details
- Title: Subtitle
- Effect of mitoquinone on liver metabolism and steatosis in obese and diabetic rats
- Creators
- Brian D. Fink - University of IowaLiping Yu - University of IowaLawrence Coppey - University of IowaAlexander Obrosov - University of IowaHanna Shevalye - University of IowaRobert J. Kerns - University of IowaMark A. Yorek - University of IowaWilliam I. Sivitz - University of Iowa
- Resource Type
- Journal article
- Publication Details
- Pharmacology research & perspectives, Vol.9(1), pp.e00701-n/a
- DOI
- 10.1002/prp2.701
- PMID
- 33547885
- PMCID
- PMC7866483
- NLM abbreviation
- Pharmacol Res Perspect
- ISSN
- 2052-1707
- eISSN
- 2052-1707
- Publisher
- John Wiley and Sons Inc
- Grant note
- Iowa Fraternal Order of the Eagles DK107339‐04; DK115256‐02 / ; Department of Veterans Affairs Iowa City Health Care System I01BX000285; RX000889‐05 / Veterans Health Administration
- Alternative title
- FINK et al
- Language
- English
- Date published
- 02/06/2021
- Academic Unit
- Pharmaceutical Sciences and Experimental Therapeutics; Fraternal Order of Eagles Diabetes Research Center; Biochemistry and Molecular Biology; Medicine Administration; Endocrinology and Metabolism; Medicinal and Natural Products Chemistry; Internal Medicine; Ophthalmology and Visual Sciences
- Record Identifier
- 9984359578402771
Metrics
11 Record Views