Journal article
Effect of olfactory bulb ablation on spread of a neurotropic coronavirus into the mouse brain
Journal of Experimental Medicine, Vol.172(4), pp.1127-1132
1990
DOI: 10.1084/jem.172.4.1127
PMID: 1698910
Abstract
Previous results suggested that, after intranasal inoculation, mouse hepatitis virus (MHV), a neurotropic coronavirus, entered the central nervous system (CNS) via the olfactory and trigeminal nerves. To prove this hypothesis, the effect of interruption of the olfactory pathway on spread of the virus was studied using in situ hybridization. Unilateral surgical ablation of this pathway prevented spread of the virus via the olfactory tract on the side of the lesion. MHV RNA could be detected, however, at distal sites on the operated side, indicating that the virus spread via well-described circuits involving the anterior commissure from the control (intact) side of the brain. Viral transport via the trigeminal nerve was not affected by removal of the olfactory bulb, showing that the surgical procedure was specific for the olfactory pathway. These results prove conclusively that MHV gains entry to the CNS via a transneuronal route, and spreads to additional sites in the brain via known neuroanatomic pathways.
Details
- Title: Subtitle
- Effect of olfactory bulb ablation on spread of a neurotropic coronavirus into the mouse brain
- Creators
- Stanley PerlmanGregory EvansAdel K Afifi - University of Iowa, Stead Family Department of Pediatrics
- Resource Type
- Journal article
- Publication Details
- Journal of Experimental Medicine, Vol.172(4), pp.1127-1132
- DOI
- 10.1084/jem.172.4.1127
- PMID
- 1698910
- NLM abbreviation
- J Exp Med
- ISSN
- 0022-1007
- eISSN
- 1540-9538
- Language
- English
- Date published
- 1990
- Academic Unit
- Microbiology and Immunology; Stead Family Department of Pediatrics; Iowa Neuroscience Institute; Infectious Disease (Pediatrics)
- Record Identifier
- 9983777346002771
Metrics
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