Journal article
Effect of silver nanoparticles associated with fluoride on the progression of root dentin caries in vitro
PloS one, Vol.18(1), pp.e0277275-e0277275
01/20/2023
DOI: 10.1371/journal.pone.0277275
PMCID: PMC9858332
PMID: 36662694
Abstract
To assess the anti-proteolytic effect and potential to inhibit dentin root caries progression of a silver nanoparticle and fluoride solution (CNanoF) in comparison to silver diamine fluoride (SDF).
48 specimens of root dentin artificial caries lesion were treated with 38% SDF, CNanoF, CNano or F (n = 6 per group). Ph cycling with demineralization and remineralization solutions simulated caries lesion progression. In addition, specimens were incubated with or without bacterial collagenase in the remineralization solution to induce dentin proteolytic degradation. Dentin degradation was assessed by weight loss rate and hydroxyproline (Hyp) release. Changes in cross-sectional microhardness, and lesion permeability and collagen integrity as determined by confocal laser scanning microscopy indicated potential for further demineralization inhibition. The effect of the solutions on the activity of metalloproteinases (MMP) -2 and -9 was also investigated. Statistical analysis consisted of ANOVA, Kruskal-Wallis, and linear mixed models with post-hoc pairwise Tukey, Dunn, and t-tests (α = 0.05).
Treatment with SDF resulted in lower weight loss rate than did other solutions, but all groups showed similar Hyp release (p = 0.183). SDF resulted in greater microhardness at superficial layers of the caries lesions (p<0.05), while there were no differences among CNanoF, CNano, and F. Lesion permeability was similar among all groups after pH cycling (p>0.05), with or without the use of collagenase (p = 0.58). No statistically significant difference was noted among solutions regarding collagen integrity after pH cycling; however, SDF-treated dentin had a significant decrease in collagen integrity when collagenase was used (p = 0.003). Interestingly, only SDF was able to completely inactivate MMP-2 and -9.
CNanoF and SDF both potentially prevent dentin degradation during caries lesion progression in vitro; however, SDF was more effective at inhibiting further tissue demineralization.
Details
- Title: Subtitle
- Effect of silver nanoparticles associated with fluoride on the progression of root dentin caries in vitro
- Creators
- Flaviana Alves Dias - Universidade Norte do ParanáCristina M P Vidal - University of IowaCarissa L Comnick - University of IowaXian Jin Xie - University of IowaSandrine Bittencourt Berger - Universidade Norte do Paraná
- Resource Type
- Journal article
- Publication Details
- PloS one, Vol.18(1), pp.e0277275-e0277275
- DOI
- 10.1371/journal.pone.0277275
- PMID
- 36662694
- PMCID
- PMC9858332
- NLM abbreviation
- PLoS One
- ISSN
- 1932-6203
- eISSN
- 1932-6203
- Grant note
- DOI: 10.13039/100005364, name: American Dental Education Association; DOI: 10.13039/501100002322, name: Coordenação de Aperfeiçoamento de Pessoal de Nível Superior
- Language
- English
- Date published
- 01/20/2023
- Academic Unit
- Preventive and Community Dentistry; Biostatistics; Operative Dentistry; Dental Research
- Record Identifier
- 9984367759802771
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