Journal article
Effect of specimen storage on absolute CD4 counts
Clinical and diagnostic laboratory immunology, Vol.1(2), pp.232-234
03/1994
DOI: 10.1128/CDLI.1.2.232-234.1994
PMID: 7496951
Abstract
We have evaluated the effect of specimen storage on absolute CD4 counts by a commercially available manual assay. This assay utilizes latex particles coated with CD4 monoclonal antibodies that are mixed with lymphocytes in whole blood. Thirty blood samples were analyzed on days 1, 2, 4, and 7 postcollection. Linear regression analysis and Pearson correlation coefficients were used to determine the relationship between the absolute CD4 count and the storage time after sample collection. There was a significant decrease in absolute CD4 counts from baseline over time, dropping 3.6% at day 2, 10.1% at day 4, and 18.8% at day 7. However, the standard error of the B coefficient was constant [SE (B) = 0.031] up to day 4, indicating that reliable estimates of the baseline CD4 counts could be made from the CD4 counts determined up to day 4 from the time of sample collection. In addition to being sample, rapid, and inexpensive, the manual assay is capable of giving a reliable absolute CD4 count after specimen storage of up to 4 days. The application of this assay in the limited facilities of developing countries' laboratories is attractive.
Details
- Title: Subtitle
- Effect of specimen storage on absolute CD4 counts
- Creators
- S Kabengera - Uganda Cancer Institute, Kampala, UgandaD HomB Jackson
- Resource Type
- Journal article
- Publication Details
- Clinical and diagnostic laboratory immunology, Vol.1(2), pp.232-234
- Publisher
- United States
- DOI
- 10.1128/CDLI.1.2.232-234.1994
- PMID
- 7496951
- ISSN
- 1071-412X
- eISSN
- 1098-6588
- Grant note
- 5D43-TW00011 / FIC NIH HHS AI 25879 / NIAID NIH HHS AI 32395 / NIAID NIH HHS
- Language
- English
- Date published
- 03/1994
- Academic Unit
- Pathology; VPMA - Administration
- Record Identifier
- 9984046913802771
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